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Validating intravascular imaging with serial optical coherence tomography and confocal fluorescence microscopy

Pier-Luc Tardif, Marie-Jeanne Bertrand, Maxime Abran, Alexandre Castonguay, Joël Lefebvre, Barbara E. Stähli, Nolwenn Merlet, Teodora Mihalache-Avram, Pascale Geoffroy, Mélanie Mecteau, David Busseuil, Feng Ni, Abedelnasser Abulrob, Éric Rhéaume, Philippe L’Allier, Jean-Claude Tardif and Frédéric Lesage

Article (2016)

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Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology.

Uncontrolled Keywords

atherosclerosis; confocal fluorescence microscopy; ex vivo three-dimensional (3D) histology; intravascular ultrasound (IVUS); near-infrared fluorescence (NIRF); optical coherence tomography (OCT); Animals; Antibodies; Artifacts; Blood Vessels; Catheters; Imaging, Three-Dimensional; Intercellular Adhesion Molecule-1; Male; Microscopy, Confocal; Microscopy, Fluorescence; Rabbits; Tomography, Optical Coherence; Antibodies; Intercellular Adhesion Molecule-1

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
2500 Electrical and electronic engineering > 2500 Electrical and electronic engineering
9000 Health sciences > 9000 Health sciences
Department: Department of Electrical Engineering
Institut de génie biomédical
PolyPublie URL: https://publications.polymtl.ca/3504/
Journal Title: International Journal of Molecular Sciences (vol. 17, no. 12)
Publisher: MDPI
DOI: 10.3390/ijms17122110
Official URL: https://doi.org/10.3390/ijms17122110
Date Deposited: 17 Dec 2018 15:53
Last Modified: 11 May 2023 03:56
Cite in APA 7: Tardif, P.-L., Bertrand, M.-J., Abran, M., Castonguay, A., Lefebvre, J., Stähli, B. E., Merlet, N., Mihalache-Avram, T., Geoffroy, P., Mecteau, M., Busseuil, D., Ni, F., Abulrob, A., Rhéaume, É., L’Allier, P., Tardif, J.-C., & Lesage, F. (2016). Validating intravascular imaging with serial optical coherence tomography and confocal fluorescence microscopy. International Journal of Molecular Sciences, 17(12), 1-12. https://doi.org/10.3390/ijms17122110


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