Validating intravascular imaging with serial optical coherence tomography and confocal fluorescence microscopy

Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology.

Uncontrolled Keywords

atherosclerosis; confocal fluorescence microscopy; ex vivo three-dimensional (3D) histology; intravascular ultrasound (IVUS); near-infrared fluorescence (NIRF); optical coherence tomography (OCT); Animals; Antibodies; Artifacts; Blood Vessels; Catheters; Imaging, Three-Dimensional; Intercellular Adhesion Molecule-1; Male; Microscopy, Confocal; Microscopy, Fluorescence; Rabbits; Tomography, Optical Coherence; Antibodies; Intercellular Adhesion Molecule-1