<  Back to the Polytechnique Montréal portal

Test-retest reliability of myelin imaging in the human spinal cord: Measurement errors versus region- and aging-induced variations

Simon Lévy, Marie-Claude Guertin, Ali Khatibi, Aviv Mezer, Kristina Martinu, Jen I. Chen, Nikola Stikov, Pierre Rainville and Julien Cohen-Adad

Article (2018)

Published Version
Terms of Use: Creative Commons Attribution .
Download (16MB)
Terms of Use: Creative Commons Attribution .
Download (378kB)
Cite this document: Lévy, S., Guertin, M.-C., Khatibi, A., Mezer, A., Martinu, K., Chen, J. I., ... Cohen-Adad, J. (2018). Test-retest reliability of myelin imaging in the human spinal cord: Measurement errors versus region- and aging-induced variations. PLOS One, 13(1). doi:10.1371/journal.pone.0189944
Show abstract Hide abstract


PURPOSE: To implement a statistical framework for assessing the precision of several quantitative MRI metrics sensitive to myelin in the human spinal cord: T1, Magnetization Transfer Ratio (MTR), saturation imposed by an off-resonance pulse (MTsat) and Macromolecular Tissue Volume (MTV). METHODS: Thirty-three healthy subjects within two age groups (young, elderly) were scanned at 3T. Among them, 16 underwent the protocol twice to assess repeatability. Statistical reliability indexes such as the Minimal Detectable Change (MDC) were compared across metrics quantified within different cervical levels and white matter (WM) sub-regions. The differences between pathways and age groups were quantified and interpreted in context of the test-retest repeatability of the measurements. RESULTS: The MDC was respectively 105.7ms, 2.77%, 0.37% and 4.08% for T1, MTR, MTsat and MTV when quantified over all WM, while the standard-deviation across subjects was 70.5ms, 1.34%, 0.20% and 2.44%. Even though particular WM regions did exhibit significant differences, these differences were on the same order as test-retest errors. No significant difference was found between age groups for all metrics. CONCLUSION: While T1-based metrics (T1 and MTV) exhibited better reliability than MT-based measurements (MTR and MTsat), the observed differences between subjects or WM regions were comparable to (and often smaller than) the MDC. This makes it difficult to determine if observed changes are due to variations in myelin content, or simply due to measurement error. Measurement error remains a challenge in spinal cord myelin imaging, but this study provides statistical guidelines to standardize the field and make it possible to conduct large-scale multi-center studies.

Uncontrolled Keywords

Adult; Aged; Aging/*metabolism; Female; Humans; Male; Middle Aged; Myelin Sheath/*metabolism; Reproducibility of Results; Spinal Cord/diagnostic imaging/*metabolism; Young Adult

Open Access document in PolyPublie
Department: Département de génie électrique
Research Center: NeuroPoly - Laboratoire de Recherche en Neuroimagerie
Funders: Canada Research Chair in Quantitative Magnetic Resonance Imaging (JCA)
Date Deposited: 17 Dec 2021 13:41
Last Modified: 13 Jan 2022 11:22
PolyPublie URL: https://publications.polymtl.ca/5000/
Document issued by the official publisher
Journal Title: PLOS One (vol. 13, no. 1)
Publisher: PLOS
Official URL: https://doi.org/10.1371/journal.pone.0189944


Total downloads

Downloads per month in the last year

Origin of downloads


Repository Staff Only