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In vivo near-infrared fluorescence imaging of atherosclerosis using local delivery of novel targeted molecular probes

Marie-Jeanne Bertrand, Maxime Abran, Foued Maafi, David Busseuil, Nolwenn Merlet, Teodora Mihalache-Avram, Pascale Geoffroy, Pier-Luc Tardif, Abedelnasser Abulrob, Mehdi Arbabi-Ghahroudi, Feng Ni, Martin Sirois, Philippe L. L'Allier, Éric Rhéaume, Frédéric Lesage et Jean-Claude Tardif

Article de revue (2019)

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Abstract

This study aimed to evaluate the feasibility and accuracy of a technique for atherosclerosis imaging using local delivery of relatively small quantities (0.04-0.4 mg/kg) of labeled-specific imaging tracers targeting ICAM-1 and unpolymerized type I collagen or negative controls in 13 rabbits with atheroma induced by balloon injury in the abdominal aorta and a 12-week high-cholesterol diet. Immediately after local infusion, in vivo intravascular ultrasonography (IVUS)-NIRF imaging was performed at different time-points over a 40-minute period. The in vivo peak NIRF signal was significantly higher in the molecular tracer-injected rabbits than in the control-injected animals (P < 0.05). Ex vivo peak NIRF signal was significantly higher in the ICAM-1 probe-injected rabbits than in controls (P = 0.04), but not in the collagen probe-injected group (P = 0.29). NIRF signal discrimination following dual-probe delivery was also shown to be feasible in a single animal and thus offers the possibility of combining several distinct biological imaging agents in future studies. This innovative imaging strategy using in vivo local delivery of low concentrations of labeled molecular tracers followed by IVUS-NIRF catheter-based imaging holds potential for detection of vulnerable human coronary artery plaques.

Sujet(s): 2500 Génie électrique et électronique > 2500 Génie électrique et électronique
5300 Biologie moléculaire > 5300 Biologie moléculaire
Département: Département de génie électrique
Institut de génie biomédical
Organismes subventionnaires: University of Montreal Endowed Research Chair in atherosclerosis, Canada Research Chair in translational and personalized medicine, NSERC CRC Chair in Optical Vascular Imaging, Instituts de recherche en santé du Canada, National Research Council of Canada - Biologics and Biomanufacturing Program
Numéro de subvention: 273578, NRCC Publication No. 53369
URL de PolyPublie: https://publications.polymtl.ca/4786/
Titre de la revue: Scientific Reports (vol. 9, no 1)
Maison d'édition: Nature
DOI: 10.1038/s41598-019-38970-4
URL officielle: https://doi.org/10.1038/s41598-019-38970-4
Date du dépôt: 07 avr. 2021 12:09
Dernière modification: 21 mai 2023 18:18
Citer en APA 7: Bertrand, M.-J., Abran, M., Maafi, F., Busseuil, D., Merlet, N., Mihalache-Avram, T., Geoffroy, P., Tardif, P.-L., Abulrob, A., Arbabi-Ghahroudi, M., Ni, F., Sirois, M., L'Allier, P. L., Rhéaume, É., Lesage, F., & Tardif, J.-C. (2019). In vivo near-infrared fluorescence imaging of atherosclerosis using local delivery of novel targeted molecular probes. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-38970-4

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