<  Back to the Polytechnique Montréal portal

In vivo near-infrared fluorescence imaging of atherosclerosis using local delivery of novel targeted molecular probes

Marie-Jeanne Bertrand, Maxime Abran, Foued Maafi, David Busseuil, Nolwenn Merlet, Teodora Mihalache-Avram, Pascale Geoffroy, Pier-Luc Tardif, Abedelnasser Abulrob, Mehdi Arbabi-Ghahroudi, Feng Ni, Martin Sirois, Philippe L. L'Allier, Éric Rhéaume, Frédéric Lesage and Jean-Claude Tardif

Article (2019)

[img]
Preview
Published Version
Terms of Use: Creative Commons Attribution.
Download (3MB)
Cite this document: Bertrand, M.-J., Abran, M., Maafi, F., Busseuil, D., Merlet, N., Mihalache-Avram, T., ... Tardif, J.-C. (2019). In vivo near-infrared fluorescence imaging of atherosclerosis using local delivery of novel targeted molecular probes. Scientific Reports, 9(1). doi:10.1038/s41598-019-38970-4
Show abstract Hide abstract

Abstract

This study aimed to evaluate the feasibility and accuracy of a technique for atherosclerosis imaging using local delivery of relatively small quantities (0.04-0.4 mg/kg) of labeled-specific imaging tracers targeting ICAM-1 and unpolymerized type I collagen or negative controls in 13 rabbits with atheroma induced by balloon injury in the abdominal aorta and a 12-week high-cholesterol diet. Immediately after local infusion, in vivo intravascular ultrasonography (IVUS)-NIRF imaging was performed at different time-points over a 40-minute period. The in vivo peak NIRF signal was significantly higher in the molecular tracer-injected rabbits than in the control-injected animals (P < 0.05). Ex vivo peak NIRF signal was significantly higher in the ICAM-1 probe-injected rabbits than in controls (P = 0.04), but not in the collagen probe-injected group (P = 0.29). NIRF signal discrimination following dual-probe delivery was also shown to be feasible in a single animal and thus offers the possibility of combining several distinct biological imaging agents in future studies. This innovative imaging strategy using in vivo local delivery of low concentrations of labeled molecular tracers followed by IVUS-NIRF catheter-based imaging holds potential for detection of vulnerable human coronary artery plaques.

Open Access document in PolyPublie
Subjects: 2500 Génie électrique et électronique > 2500 Génie électrique et électronique
5300 Biologie moléculaire > 5300 Biologie moléculaire
Department: Département de génie électrique
Institut de génie biomédical
Research Center: Non applicable
Funders: University of Montreal Endowed Research Chair in atherosclerosis, Canada Research Chair in translational and personalized medicine, NSERC CRC Chair in Optical Vascular Imaging, Instituts de recherche en santé du Canada, National Research Council of Canada - Biologics and Biomanufacturing Program
Grant number: 273578, NRCC Publication No. 53369
Date Deposited: 07 Apr 2021 12:09
Last Modified: 08 Apr 2021 10:42
PolyPublie URL: https://publications.polymtl.ca/4786/
Document issued by the official publisher
Journal Title: Scientific Reports (vol. 9, no. 1)
Publisher: Nature
Official URL: https://doi.org/10.1038/s41598-019-38970-4

Statistics

Total downloads

Downloads per month in the last year

Origin of downloads

Dimensions

Repository Staff Only