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Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing

Jimmy Gaudreault, Benoît Liberelle, Yves Durocher, Olivier Henry and Gregory De Crescenzo

Article (2021)

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Cite this document: Gaudreault, J., Liberelle, B., Durocher, Y., Henry, O. & De Crescenzo, G. (2021). Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing. Scientific Reports, 11. doi:10.1038/s41598-021-83268-z
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Abstract

Surface plasmon resonance-based biosensors have been extensively applied to the characterization of the binding kinetics between purified (bio)molecules, thanks to robust data analysis techniques. However, data analysis for solutions containing multiple interactants is still at its infancy. We here present two algorithms for (1) the reliable and accurate determination of the kinetic parameters of N interactants present at different ratios in N mixtures and (2) the estimation of the ratios of each interactant in a given mixture, assuming that their kinetic parameters are known. Both algorithms assume that the interactants compete to bind to an immobilized ligand in a 1:1 fashion and necessitate prior knowledge of the total concentration of all interactants combined. The effectiveness of these two algorithms was experimentally validated with a model system corresponding to mixtures of four small molecular weight drugs binding to an immobilized protein. This approach enables the in-depth characterization of mixtures using SPR, which may be of considerable interest for many drug discovery or development applications, notably for protein glycovariant analysis.

Uncontrolled Keywords

Assay systems, Biochemistry, Biophysical chemistry, Pharmaceutics

Open Access document in PolyPublie
Subjects: 1800 Génie chimique > 1800 Génie chimique
1800 Génie chimique > 1802 Génie biochimique
Department: Département de génie chimique
Research Center: Non applicable
Funders: CRSNG/NSERC, TransMedTech Institute (NanoBio Technology Platform), Canada First Research Excellence Fund
Date Deposited: 16 May 2022 16:50
Last Modified: 17 May 2022 01:20
PolyPublie URL: https://publications.polymtl.ca/9293/
Document issued by the official publisher
Journal Title: Scientific Reports (vol. 11)
Publisher: Springer Nature
Official URL: https://doi.org/10.1038/s41598-021-83268-z

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