<  Back to the Polytechnique Montréal portal

Carotid calcification in mice: a new model to study the effects of arterial stiffness on the brain

Nataliya Sadekova, Dominic Vallerand, Edgar Guevara, Frédéric Lesage and Hélène Girouard

Article (2013)

Open Acess document in PolyPublie and at official publisher
Open Access to the full text of this document
Published Version
Terms of Use: All rights reserved
Download (937kB)
Show abstract
Hide abstract


BACKGROUND: Arterial stiffness has been identified as an important risk factor for cognitive decline. However, its effects on the brain's health are unknown, and there is no animal model available to study the precise impact of arterial stiffness on the brain. Therefore, the objective of the study was to develop and characterize a new model specific to arterial stiffness in order to study its effects on the brain. METHODS AND RESULTS: Calcium chloride (CaCl2) was applied to carotid arteries of mice, inducing an increase in collagen distribution and intima-media thickness, a fragmentation of elastin, a decrease in arterial compliance and distensibility, and an increase in cerebral blood flow pulsatility (n=3 to 11). Calcium deposits were only present at the site of CaCl2 application, and there was no increase in systemic blood pressure or change in vessel radius making this model specific for arterial stiffness. The effects of carotid stiffness were then assessed in the brain. Carotid calcification induced an increase in the production of cerebral superoxide anion and neurodegeneration, detected with Fluoro-Jade B staining, in the hippocampus (n=3 to 5), a key region for memory and cognition. CONCLUSIONS: A new model of arterial stiffness based on carotid calcification was developed and characterized. This new model meets all the characteristics of arterial stiffness, and its specificity allows the study of the effects of arterial stiffness on the brain.

Uncontrolled Keywords

Animals; Brain/*blood supply; Carotid Arteries/*pathology/physiopathology; Cerebrovascular Circulation; Disease Models, Animal; Male; Mice; Mice, Inbred C57BL; Regional Blood Flow; Vascular Calcification/*complications; *Vascular Stiffness; arterial stiffness; brain; calcium chloride; carotid

Department: Department of Electrical Engineering
Funders: Fonds de la Recherche en Santé du Québec (FRSQ), Heart and Stroke Foundation of Canada (HSFC), Canadian Foundation for Innovation, and the Canadian Institutes of Health Research, Société Québécoise d'Hypertension Artérielle
PolyPublie URL: https://publications.polymtl.ca/5120/
Journal Title: Journal of the American Heart Association (vol. 2, no. 3)
Publisher: Wiley‐Blackwell
DOI: 10.1161/jaha.113.000224
Official URL: https://doi.org/10.1161/jaha.113.000224
Date Deposited: 07 Apr 2022 11:23
Last Modified: 11 May 2023 22:02
Cite in APA 7: Sadekova, N., Vallerand, D., Guevara, E., Lesage, F., & Girouard, H. (2013). Carotid calcification in mice: a new model to study the effects of arterial stiffness on the brain. Journal of the American Heart Association, 2(3), e000224. https://doi.org/10.1161/jaha.113.000224


Total downloads

Downloads per month in the last year

Origin of downloads


Repository Staff Only

View Item View Item