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Multiplexed plasmonic nano-labeling for bioimaging of cytological stained samples

Paule Marcoux-Valiquette, Cécile Darviot, Lu Wang, Andrée-Anne Grosset, Morteza Hasanzadeh Kafshgari, Mirela Birela, Sergiy Patskovsky, Dominique Trudel and Michel Meunier

Article (2021)

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Reliable cytopathological diagnosis requires new methods and approaches for the rapid and accurate determination of all cell types. This is especially important when the number of cells is limited, such as in the cytological samples of fine-needle biopsy. Immunoplasmonic-multiplexedlabeling may be one of the emerging solutions to such problems. However, to be accepted and used by the practicing pathologists, new methods must be compatible and complementary with existing cytopathology approaches where counterstaining is central to the correct interpretation of immunolabeling. In addition, the optical detection and imaging setup for immunoplasmonicmultiplexed-labeling must be implemented on the same cytopathological microscope, not interfere with standard H&E imaging, and operate as a second easy-to-use imaging method. In this article, we present multiplex imaging of four types of nanoplasmonic markers on two types of H&E-stained cytological specimens (formalin-fixed paraffin embedded and non-embedded adherent cancer cells) using a specially designed adapter for SI dark-field microscopy. The obtained results confirm the effectiveness of the proposed optical method for quantitative and multiplex identification of various plasmonic NPs, and the possibility of using immunoplasmonic-multiplexed-labeling for cytopathological diagnostics.

Uncontrolled Keywords

cytopathological cancer diagnosis; paraffin-embedded cytological tissues; gold nanoparticles; gold-silver alloy nanoparticles; plasmonic biomarkers; immunoplasmonic-multiplexed-labeling; side-illumination microscopy; bioimaging

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
1900 Biomedical engineering > 1901 Biomedical technology
3100 Physics > 3100 Physics
3100 Physics > 3101 Atomic and molecular studies
3100 Physics > 3110 Optics (see also Photon devices, 2505)
Department: Department of Engineering Physics
Funders: Onco-Tech Competition Fund, TransMedTech Institute Medical Technology Innovation Fund
Grant number: 293742, 0049
PolyPublie URL: https://publications.polymtl.ca/9370/
Journal Title: Cancers (vol. 13, no. 14)
Publisher: MDPI
DOI: 10.3390/cancers13143509
Official URL: https://doi.org/10.3390/cancers13143509
Date Deposited: 16 Aug 2023 12:13
Last Modified: 08 Apr 2024 11:54
Cite in APA 7: Marcoux-Valiquette, P., Darviot, C., Wang, L., Grosset, A.-A., Hasanzadeh Kafshgari, M., Birela, M., Patskovsky, S., Trudel, D., & Meunier, M. (2021). Multiplexed plasmonic nano-labeling for bioimaging of cytological stained samples. Cancers, 13(14), 3509 (10 pages). https://doi.org/10.3390/cancers13143509


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