Hydrological and physicochemical parameters associated with SARS-CoV-2 and pepper mild mottle virus wastewater concentrations for a large-combined sewer system

During COVID-19, surveillance of SARS-CoV-2 in wastewater has been a promising tool for tracking viral infection at the community level. However, in addition to the shedding rates within the community, SARS-CoV-2 concentrations in raw wastewater are influenced by several environmental factors. This study investigated the effects of wastewater characteristics on the viral quantification of SARS-CoV-2 and pepper mild mottle virus (PMMoV) for a large wastewater system with combined sewers. Principal component analysis illustrated that water temperature negatively correlates with SARS-CoV-2 and PMMoV in wastewater, but flow rate and EC are highly correlated with SARS-CoV-2 in spring and winter. The normalization using EC enhanced the correlation with clinical data compared to normalization using pH, flow rate, and raw SARS-CoV-2. The normalization using PMMoV reduced the correlation with clinical data. Multiple linear and random forest (RF) applied to predict the concentrations of SARS-CoV-2 in wastewater, given the confirmed cases and physicochemical parameters. RF regression was the best model to predict SARS-CoV-2 in wastewater (R2=0.8), with the most important variables being the confirmed cases followed by water temperature. RF model is a potent predictor of the presence of SARS-CoV-2 in wastewater. This enhances the degree of reliability between community outbreaks and SARS-CoV-2 monitoring.