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Dual-tracer background subtraction approach for fluorescent molecular tomography

Kenneth M. Tichauer, Robert W. Holt, Fadi El-Ghussein, Scott C. Davis, Kimberley S. Samkoe, Jason R. Gunn, Frédéric Leblond and Brian W. Pogue

Article (2013)

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Abstract

Diffuse fluorescence tomography requires high contrast-to-background ratios to accurately reconstruct inclusions of interest. This is a problem when imaging the uptake of fluorescently labeled molecularly targeted tracers in tissue, which can result in high levels of heterogeneously distributed background uptake. We present a dual-tracer background subtraction approach, wherein signal from the uptake of an untargeted tracer is subtracted from targeted tracer signal prior to image reconstruction, resulting in maps of targeted tracer binding. The approach is demonstrated in simulations, a phantom study, and in a mouse glioma imaging study, demonstrating substantial improvement over conventional and homogenous background subtraction image reconstruction approaches.

Uncontrolled Keywords

Animals; Computer Simulation; Glioma/metabolism/pathology; Humans; Image Processing, Computer-Assisted/*methods; Mice; *Models, Biological; Molecular Imaging/*methods; Neoplasm Transplantation; Phantoms, Imaging; *Radioactive Tracers; Reproducibility of Results; Signal Processing, Computer-Assisted; Spectrometry, Fluorescence/*methods; Tomography, X-Ray Computed/instrumentation/*methods

Subjects: 1900 Biomedical engineering > 1901 Biomedical technology
3100 Physics > 3108 Experimental methods and instrumentation
Department: Department of Engineering Physics
Funders: NCI, CIHR
Grant number: R01CA120368, R01CA109558, K25 CA138578
PolyPublie URL: https://publications.polymtl.ca/5151/
Journal Title: Journal of Biomedical Optics (vol. 18, no. 1)
Publisher: SPIE
DOI: 10.1117/1.jbo.18.1.016003
Official URL: https://doi.org/10.1117/1.jbo.18.1.016003
Date Deposited: 07 Apr 2022 16:09
Last Modified: 08 Apr 2024 18:20
Cite in APA 7: Tichauer, K. M., Holt, R. W., El-Ghussein, F., Davis, S. C., Samkoe, K. S., Gunn, J. R., Leblond, F., & Pogue, B. W. (2013). Dual-tracer background subtraction approach for fluorescent molecular tomography. Journal of Biomedical Optics, 18(1), 16003. https://doi.org/10.1117/1.jbo.18.1.016003

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