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A data-independent methodology for the structural characterization of microcystins and anabaenopeptins leading to the identification of four new congeners

Audrey Roy-Lachapelle, Morgan Solliec, Sébastien Sauvé and Christian Gagnon

Article (2019)

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Abstract

Toxin-producing cyanobacteria are responsible for the presence of hundreds of bioactive compounds in aquatic environments undergoing increasing eutrophication. The identification of cyanotoxins is still emerging, due to the great diversity of potential congeners, yet high-resolution mass spectrometry (HRMS) has the potential to deepen this knowledge in aquatic environments. In this study, high-throughput and sensitive on-line solid-phase extraction ultra-high performance liquid chromatography (SPE-UHPLC) coupled to HRMS was applied to a data-independent acquisition (DIA) workflow for the suspect screening of cyanopeptides, including microcystin and anabaenopeptin toxin classes. The unambiguous characterization of 11 uncommon cyanopeptides was possible using a characterization workflow through extensive analysis of fragmentation patterns. This method also allowed the characterization of four unknown cyanotoxins ([Leu(1), Ser(7)] MC-HtyR, [Asp(3)]MC-RHar, AP731, and AP803). The quantification of 17 common cyanotoxins along with the semi-quantification of the characterized uncommon cyanopeptides resulted with the identification of 23 different cyanotoxins in 12 lakes in Canada, United Kingdom and France. The concentrations of the compounds varied between 39 and 41,000 ng L(-1). To our knowledge, this is the first DIA method applied for the suspect screening of two families of cyanopeptides simultaneously. Moreover, this study shows the great diversity of cyanotoxins in lake water cyanobacterial blooms, a growing concern in aquatic systems.

Uncontrolled Keywords

Lc-hrms; anabaenopeptins; cyanobacteria; cyanotoxins; data-independent acquisition; microcystins; non-target screening; structural characterization; suspect screening

Subjects: 5200 Microbiology > 5200 Microbiology
Department: Department of Civil, Geological and Mining Engineering
Research Center: CIEP - Industrial Chair on Drinking Water
Funders: St. Lawrence Action Plan, Génome Québec, Genome Canada, LSARP Program for the ATRAPP project (Algal Blooms, Treatment, Risk Assessment, Prediction and Prevention through Genomics)
PolyPublie URL: https://publications.polymtl.ca/5118/
Journal Title: Toxins (vol. 11, no. 11)
Publisher: MDPI
DOI: 10.3390/toxins11110619
Official URL: https://doi.org/10.3390/toxins11110619
Date Deposited: 05 Jul 2022 12:37
Last Modified: 10 Apr 2024 05:03
Cite in APA 7: Roy-Lachapelle, A., Solliec, M., Sauvé, S., & Gagnon, C. (2019). A data-independent methodology for the structural characterization of microcystins and anabaenopeptins leading to the identification of four new congeners. Toxins, 11(11), 619 (21 pages). https://doi.org/10.3390/toxins11110619

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