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A new method using Raman spectroscopy for in vivo targeted brain cancer tissue biopsy

Joannie Desroches, Michael Jermyn, Michael Pinto, Fabien Picot, Marie-Andrée Tremblay, Sami Obaid, Eric Marple, Kirk Urmey, Dominique Trudel, Gilles Soulez, Marie-Christine Guiot, Brian C. Wilson, Kevin Petrecca and Frédéric Leblond

Article (2018)

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Cite this document: Desroches, J., Jermyn, M., Pinto, M., Picot, F., Tremblay, M.-A., Obaid, S., ... Leblond, F. (2018). A new method using Raman spectroscopy for in vivo targeted brain cancer tissue biopsy. Scientific Reports, 8. doi:10.1038/s41598-018-20233-3
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Abstract

Modern cancer diagnosis requires histological, molecular, and genomic tumor analyses. Tumor sampling is often achieved using a targeted needle biopsy approach. Targeting errors and cancer heterogeneity causing inaccurate sampling are important limitations of this blind technique leading to non-diagnostic or poor quality samples, and the need for repeated biopsies pose elevated patient risk. An optical technology that can analyze the molecular nature of the tissue prior to harvesting could improve cancer targeting and mitigate patient risk. Here we report on the design, development, and validation of an in situ intraoperative, label-free, cancer detection system based on high wavenumber Raman spectroscopy. This optical detection device was engineered into a commercially available biopsy system allowing tumor analysis prior to tissue harvesting without disrupting workflow. Using a dual validation approach we show that high wavenumber Raman spectroscopy can detect human dense cancer with >60% cancer cells in situ during surgery with a sensitivity and specificity of 80% and 90%, respectively. We also demonstrate for the first time the use of this system in a swine brain biopsy model. These studies set the stage for the clinical translation of this optical molecular imaging method for high yield and safe targeted biopsy.

Uncontrolled Keywords

Adult; Aged; Animals; Biopsy; Brain Neoplasms/*diagnosis/*pathology; Female; Humans; Male; Middle Aged; Spectrum Analysis, Raman/*methods; Swine

Open Access document in PolyPublie
Subjects: 3100 Physique > 3100 Physique
3100 Physique > 3110 Optique (voir aussi Dispositifs photoniques, 2505)
Department: Département de génie physique
Research Center: Non applicable
Funders: CRSNG/NSERC - New Researchers and Discovery Grant, CRSNG/NSERC - Collaborative Health Research Program, Mitacs Accelerate PhD Fellowship with Medtronic
Date Deposited: 16 Aug 2021 13:35
Last Modified: 17 Aug 2021 01:20
PolyPublie URL: https://publications.polymtl.ca/4843/
Document issued by the official publisher
Journal Title: Scientific Reports (vol. 8)
Publisher: Nature
Official URL: https://doi.org/10.1038/s41598-018-20233-3

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