<  Back to the Polytechnique Montréal portal

A new method using Raman spectroscopy for in vivo targeted brain cancer tissue biopsy

Joannie Desroches, Michael Jermyn, Michael Pinto, Fabien Picot, Marie-Andrée Tremblay, Sami Obaid, Eric Marple, Kirk Urmey, Dominique Trudel, Gilles Soulez, Marie-Christine Guiot, Brian C. Wilson, Kevin Petrecca and Frédéric Leblond

Article (2018)

Open Acess document in PolyPublie and at official publisher
[img]
Preview
Open Access to the full text of this document
Published Version
Terms of Use: Creative Commons Attribution
Download (588kB)
Show abstract
Hide abstract

Abstract

Modern cancer diagnosis requires histological, molecular, and genomic tumor analyses. Tumor sampling is often achieved using a targeted needle biopsy approach. Targeting errors and cancer heterogeneity causing inaccurate sampling are important limitations of this blind technique leading to non-diagnostic or poor quality samples, and the need for repeated biopsies pose elevated patient risk. An optical technology that can analyze the molecular nature of the tissue prior to harvesting could improve cancer targeting and mitigate patient risk. Here we report on the design, development, and validation of an in situ intraoperative, label-free, cancer detection system based on high wavenumber Raman spectroscopy. This optical detection device was engineered into a commercially available biopsy system allowing tumor analysis prior to tissue harvesting without disrupting workflow. Using a dual validation approach we show that high wavenumber Raman spectroscopy can detect human dense cancer with >60% cancer cells in situ during surgery with a sensitivity and specificity of 80% and 90%, respectively. We also demonstrate for the first time the use of this system in a swine brain biopsy model. These studies set the stage for the clinical translation of this optical molecular imaging method for high yield and safe targeted biopsy.

Uncontrolled Keywords

Adult; Aged; Animals; Biopsy; Brain Neoplasms/*diagnosis/*pathology; Female; Humans; Male; Middle Aged; Spectrum Analysis, Raman/*methods; Swine

Subjects: 3100 Physics > 3100 Physics
3100 Physics > 3110 Optics (see also Photon devices, 2505)
Department: Department of Engineering Physics
Funders: CRSNG/NSERC - New Researchers and Discovery Grant, CRSNG/NSERC - Collaborative Health Research Program, Mitacs Accelerate PhD Fellowship with Medtronic
PolyPublie URL: https://publications.polymtl.ca/4843/
Journal Title: Scientific Reports (vol. 8, no. 1)
Publisher: Nature
DOI: 10.1038/s41598-018-20233-3
Official URL: https://doi.org/10.1038/s41598-018-20233-3
Date Deposited: 16 Aug 2021 13:35
Last Modified: 06 Apr 2024 09:45
Cite in APA 7: Desroches, J., Jermyn, M., Pinto, M., Picot, F., Tremblay, M.-A., Obaid, S., Marple, E., Urmey, K., Trudel, D., Soulez, G., Guiot, M.-C., Wilson, B. C., Petrecca, K., & Leblond, F. (2018). A new method using Raman spectroscopy for in vivo targeted brain cancer tissue biopsy. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-20233-3

Statistics

Total downloads

Downloads per month in the last year

Origin of downloads

Dimensions

Repository Staff Only

View Item View Item