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Fluorescence hyperspectral imaging for live monitoring of multiple spheroids in microfluidic chips

Amélie St-Georges-Robillard, Mathieu Masse, Maxime Cahuzac, Mathias Strupler, Bishnubrata Patra, Adriana Mari Orimoto, Jennifer Kendall-Dupont, Benjamin Péant, Anne-Marie Mes-Masson, Frédéric Leblond and Thomas Gervais

Article (2018)

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Abstract

Tumor spheroids represent a realistic 3D in vitro cancer model because they provide a missing link between monolayer cell culture and live tissues. While microfluidic chips can easily form and assay thousands of spheroids simultaneously, few commercial instruments are available to analyze this massive amount of data. Available techniques to measure spheroid response to external stimuli, such as confocal imaging and flow cytometry, are either not appropriate for 3D cultures, or destructive. We designed a wide-field hyperspectral imaging system to analyze multiple spheroids trapped in a microfluidic chip in a single acquisition. The system and its fluorescence quantification algorithm were assessed using liquid phantoms mimicking spheroid optical properties. Spectral unmixing was tested on three overlapping spectral entities. Hyperspectral images of co-culture spheroids expressing two fluorophores were compared with confocal microscopy and spheroid growth was measured over time. The system can spectrally analyze multiple fluorescent markers simultaneously and allows multiple time-points assays, providing a fast and versatile solution for analyzing lab on a chip devices.

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
1900 Biomedical engineering > 1901 Biomedical technology
3100 Physics > 3113 Biophysics
Department: Department of Engineering Physics
Institut de génie biomédical
Funders: NSERC / CRSNG, Canada Foundation for Innovation, Cancer Research Society, Ovarian Cancer Canada, CMC Microsystems, Fonds de recherche du Québec – Nature et technologies
Grant number: 20103
PolyPublie URL: https://publications.polymtl.ca/40755/
Journal Title: Analyst (vol. 143, no. 16)
Publisher: The Royal Society of Chemistry
DOI: 10.1039/c8an00536b
Official URL: https://doi.org/10.1039/c8an00536b
Date Deposited: 18 Apr 2023 15:03
Last Modified: 29 Sep 2024 05:11
Cite in APA 7: St-Georges-Robillard, A., Masse, M., Cahuzac, M., Strupler, M., Patra, B., Orimoto, A. M., Kendall-Dupont, J., Péant, B., Mes-Masson, A.-M., Leblond, F., & Gervais, T. (2018). Fluorescence hyperspectral imaging for live monitoring of multiple spheroids in microfluidic chips. Analyst, 143(16), 3829-3840. https://doi.org/10.1039/c8an00536b

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