Cong Zhang, Samuel Bélanger, Philippe Pouliot and Frédéric Lesage
Article (2015)
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Abstract
In this work a method for measuring brain oxygen partial pressure with confocal phosphorescence lifetime microscopy system is reported. When used in conjunction with a dendritic phosphorescent probe, Oxyphor G4, this system enabled minimally invasive measurements of oxygen partial pressure (pO2) in cerebral tissue with high spatial and temporal resolution during 4-AP induced epileptic seizures. Investigating epileptic events, we characterized the spatio-temporal distribution of the "initial dip" in pO2 near the probe injection site and along nearby arterioles. Our results reveal a correlation between the percent change in the pO2 signal during the "initial dip" and the duration of seizure-like activity, which can help localize the epileptic focus and predict the length of seizure.
Uncontrolled Keywords
4-Aminopyridine; Angiography; Animals; Brain; Epilepsy; Luminescent Measurements; Male; Mice, Inbred C57BL; Microscopy; Oxygen; Partial Pressure; Seizures; Temperature; 4-Aminopyridine; Oxygen
| Subjects: |
2500 Electrical and electronic engineering > 2500 Electrical and electronic engineering 9000 Health sciences > 9000 Health sciences |
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| Department: | Department of Electrical Engineering |
| Funders: | Canadian Institutes of Health Research, Réseau de Bioimagerie du Québec (RBIQ), Chinese Science Foundation (CSC) |
| Grant number: | RN299166 |
| PolyPublie URL: | https://publications.polymtl.ca/3486/ |
| Journal Title: | PLOS One (vol. 10, no. 8) |
| Publisher: | PLOS |
| DOI: | 10.1371/journal.pone.0135536 |
| Official URL: | https://doi.org/10.1371/journal.pone.0135536 |
| Date Deposited: | 23 Nov 2018 10:29 |
| Last Modified: | 26 Sep 2024 05:02 |
| Cite in APA 7: | Zhang, C., Bélanger, S., Pouliot, P., & Lesage, F. (2015). Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy. PLOS One, 10(8). https://doi.org/10.1371/journal.pone.0135536 |
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