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Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy

Cong Zhang, Samuel Bélanger, Philippe Pouliot, Frédéric Lesage

Article (2015)

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Abstract

In this work a method for measuring brain oxygen partial pressure with confocal phosphorescence lifetime microscopy system is reported. When used in conjunction with a dendritic phosphorescent probe, Oxyphor G4, this system enabled minimally invasive measurements of oxygen partial pressure (pO2) in cerebral tissue with high spatial and temporal resolution during 4-AP induced epileptic seizures. Investigating epileptic events, we characterized the spatio-temporal distribution of the "initial dip" in pO2 near the probe injection site and along nearby arterioles. Our results reveal a correlation between the percent change in the pO2 signal during the "initial dip" and the duration of seizure-like activity, which can help localize the epileptic focus and predict the length of seizure.

Uncontrolled Keywords

4-Aminopyridine; Angiography; Animals; Brain; Epilepsy; Luminescent Measurements; Male; Mice, Inbred C57BL; Microscopy; Oxygen; Partial Pressure; Seizures; Temperature; 4-Aminopyridine; Oxygen

Subjects: 2500 Electrical and electronic engineering > 2500 Electrical and electronic engineering
9000 Health sciences > 9000 Health sciences
Department: Department of Electrical Engineering
Funders: Canadian Institutes of Health Research, Réseau de Bioimagerie du Québec (RBIQ), Chinese Science Foundation (CSC)
Grant number: RN299166
PolyPublie URL: https://publications.polymtl.ca/3486/
Journal Title: PLOS One (vol. 10, no. 8)
Publisher: PLOS
DOI: 10.1371/journal.pone.0135536
Official URL: https://doi.org/10.1371/journal.pone.0135536
Date Deposited: 23 Nov 2018 10:29
Last Modified: 10 Nov 2022 14:01
Cite in APA 7: Zhang, C., Bélanger, S., Pouliot, P., & Lesage, F. (2015). Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy. PLOS One, 10(8). https://doi.org/10.1371/journal.pone.0135536

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