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Restoration of physiological levels of uric acid and ascorbic acid reroutes the metabolism of stored red blood cells

Manon Bardyn, Jingkui Chen, Michaël Dussiot, David Crettaz, Lucas Schmid, Emmanuel Längst, Pascal Amireault, Jean-Daniel Tissot, Mario Jolicoeur and Michel Prudent

Article (2020)

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Abstract

After blood donation, the red blood cells (RBCs) for transfusion are generally isolated by centrifugation and then filtrated and supplemented with additive solution. The consecutive changes of the extracellular environment participate to the occurrence of storage lesions. In this study, the hypothesis is that restoring physiological levels of uric and ascorbic acids (major plasmatic antioxidants) might correct metabolism defects and protect RBCs from the very beginning of the storage period, to maintain their quality. Leukoreduced CPD-SAGM RBC concentrates were supplemented with 416 µM uric acid and 114 µM ascorbic acid and stored during six weeks at 4 °C. Different markers, i.e., haematological parameters, metabolism, sensitivity to oxidative stress, morphology and haemolysis were analyzed. Quantitative metabolomic analysis of targeted intracellular metabolites demonstrated a direct modification of several metabolite levels following antioxidant supplementation. No significant differences were observed for the other markers. In conclusion, the results obtained show that uric and ascorbic acids supplementation partially prevented the metabolic shift triggered by plasma depletion that occurs during the RBC concentrate preparation. The treatment directly and indirectly sustains the antioxidant protective system of the stored RBCs.

Uncontrolled Keywords

red blood cell; blood storage; transfusion; metabolomics; antioxidant; uric acid; ascorbic acid

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
1800 Chemical engineering > 1802 Biochemical engineering
1800 Chemical engineering > 1808 Rheology and processing
Department: Department of Chemical Engineering
Funders: CETRASA foundation
PolyPublie URL: https://publications.polymtl.ca/9411/
Journal Title: Metabolites (vol. 10, no. 6)
Publisher: MDPI
DOI: 10.3390/metabo10060226
Official URL: https://doi.org/10.3390/metabo10060226
Date Deposited: 16 Aug 2023 14:05
Last Modified: 10 Apr 2024 03:22
Cite in APA 7: Bardyn, M., Chen, J., Dussiot, M., Crettaz, D., Schmid, L., Längst, E., Amireault, P., Tissot, J.-D., Jolicoeur, M., & Prudent, M. (2020). Restoration of physiological levels of uric acid and ascorbic acid reroutes the metabolism of stored red blood cells. Metabolites, 10(6), 226 (18 pages). https://doi.org/10.3390/metabo10060226

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