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On the use of surface plasmon resonance biosensing to understand IgG-FcγR interactions

Catherine Forest-Nault, Jimmy Gaudreault, Olivier Henry, Yves Durocher and Gregory De Crescenzo

Article (2021)

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Surface plasmon resonance (SPR)-based optical biosensors offer real-time and label-free analysis of protein interactions, which has extensively contributed to the discovery and development of therapeutic monoclonal antibodies (mAbs). As the biopharmaceutical market for these biologics and their biosimilars is rapidly growing, the role of SPR biosensors in drug discovery and quality assessment is becoming increasingly prominent. One of the critical quality attributes of mAbs is the N-glycosylation of their Fc region. Other than providing stability to the antibody, the Fc N-glycosylation influences immunoglobulin G (IgG) interactions with the Fcγ receptors (FcγRs), modulating the immune response. Over the past two decades, several studies have relied on SPR-based assays to characterize the influence of N-glycosylation upon the IgG-FcγR interactions. While these studies have unveiled key information, many conclusions are still debated in the literature. These discrepancies can be, in part, attributed to the design of the reported SPR-based assays as well as the methodology applied to SPR data analysis. In fact, the SPR biosensor best practices have evolved over the years, and several biases have been pointed out in the development of experimental SPR protocols. In parallel, newly developed algorithms and data analysis methods now allow taking into consideration complex biomolecular kinetics. In this review, we detail the use of different SPR biosensing approaches for characterizing the IgG-FcγR interactions, highlighting their merit and inherent experimental complexity. Furthermore, we review the latest SPR-derived conclusions on the influence of the N-glycosylation upon the IgG-FcγR interactions and underline the differences and similarities across the literature. Finally, we explore new avenues taking advantage of novel computational analysis of SPR results as well as the latest strategies to control the glycoprofile of mAbs during production, which could lead to a better understanding and modelling of the IgG-FcγRs interactions.

Uncontrolled Keywords

Fcγ receptors; surface plasmon resonance (SPR); monoclonal antibodies (mAbs); N-glycosylation; SPR data analysis

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
1800 Chemical engineering > 1802 Biochemical engineering
1900 Biomedical engineering > 1900 Biomedical engineering
Department: Department of Chemical Engineering
Funders: GRSNG / NSERC - NSERC-CREATE PrEEmiuM program, Trans-MedTech Institute (NanoBio Technology Platform), Canada First Research Excellence Fund
PolyPublie URL: https://publications.polymtl.ca/9393/
Journal Title: International Journal of Molecular Sciences (vol. 22, no. 12)
Publisher: MDPI
DOI: 10.3390/ijms22126616
Official URL: https://doi.org/10.3390/ijms22126616
Date Deposited: 27 Apr 2023 15:07
Last Modified: 07 Apr 2024 03:05
Cite in APA 7: Forest-Nault, C., Gaudreault, J., Henry, O., Durocher, Y., & De Crescenzo, G. (2021). On the use of surface plasmon resonance biosensing to understand IgG-FcγR interactions. International Journal of Molecular Sciences, 22(12), 6616 (27 pages). https://doi.org/10.3390/ijms22126616


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