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Microdissected tissue vs tissue slices - a comparative study of tumor explant models cultured on-chip and off-chip

Dina Dorrigiv, Kayla Simeone, Laudine Communal, Jennifer Kendall-Dupont, Amélie St-Georges-Robillard, Benjamin Péant, Euridice Carmona, Anne-Marie Mes-Masson and Thomas Gervais

Article (2021)

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Abstract

Predicting patient responses to anticancer drugs is a major challenge both at the drug development stage and during cancer treatment. Tumor explant culture platforms (TECPs) preserve the native tissue architecture and are well-suited for drug response assays. However, tissue longevity in these models is relatively low. Several methodologies have been developed to address this issue, although no study has compared their efficacy in a controlled fashion. We investigated the effect of two variables in TECPs, specifically, the tissue size and culture vessel on tissue survival using micro-dissected tumor tissue (MDT) and tissue slices which were cultured in microfluidic chips and plastic well plates. Tumor models were produced from ovarian and prostate cancer cell line xenografts and were matched in terms of the specimen, total volume of tissue, and respective volume of medium in each culture system. We examined morphology, viability, and hypoxia in the various tumor models. Our observations suggest that the viability and proliferative capacity of MDTs were not affected during the time course of the experiments. In contrast, tissue slices had reduced proliferation and showed increased cell death and hypoxia under both culture conditions. Tissue slices cultured in microfluidic devices had a lower degree of hypoxia compared to those in 96-well plates. Globally, our results show that tissue slices have lower survival rates compared to MDTs due to inherent diffusion limitations, and that microfluidic devices may decrease hypoxia in tumor models.

Uncontrolled Keywords

cancer treatment; drug screening assays; tumor explant culture platform; hypoxia; ex vivo model

Additional Information: The data associated with the raw experimental data have been deposited in the Scholar Portal Dataverse: Microdissected tissue vs. tissue slices—A comparative study of two tumor explant models cultured on-chip and off-chip. (https://doi.org/10.5683/SP2/KNRWND, uploaded on 5 August 2021). All other data are contained in the article and supplementary material.
Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
1900 Biomedical engineering > 1901 Biomedical technology
1900 Biomedical engineering > 1902 Biomedical materials
3100 Physics > 3100 Physics
3100 Physics > 3101 Atomic and molecular studies
Department: Department of Engineering Physics
Institut de génie biomédical
Funders: CRSNG / NSERC, Fond de recherche Québec—Santé (FRQS), Canada First Research Excellence Fund, Canadian Cancer Society Research Institute (CCSRI), Cancer Research Society (CRS), Institut du cancer de Montréal - Canderel fund, Ovarian Cancer Canada (OCC), Prostate Cancer Canada (PCC), Canadian Urological Oncology Group (CUOG)
PolyPublie URL: https://publications.polymtl.ca/9372/
Journal Title: Cancers (vol. 13, no. 16)
Publisher: MDPI
DOI: 10.3390/cancers13164208
Official URL: https://doi.org/10.3390/cancers13164208
Date Deposited: 16 Aug 2023 13:33
Last Modified: 08 Apr 2024 15:48
Cite in APA 7: Dorrigiv, D., Simeone, K., Communal, L., Kendall-Dupont, J., St-Georges-Robillard, A., Péant, B., Carmona, E., Mes-Masson, A.-M., & Gervais, T. (2021). Microdissected tissue vs tissue slices - a comparative study of tumor explant models cultured on-chip and off-chip. Cancers, 13(16), 4208 (14 pages). https://doi.org/10.3390/cancers13164208

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