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Characterization and optimization of the production of adeno-associated viral vectors using a baculovirus expression vector/insect cell system

Marc Gordon Aucoin

PhD thesis (2007)

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Cite this document: Aucoin, M. G. (2007). Characterization and optimization of the production of adeno-associated viral vectors using a baculovirus expression vector/insect cell system (PhD thesis, École Polytechnique de Montréal). Retrieved from https://publications.polymtl.ca/8052/
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Abstract

Critical assessment of current adeno-associated viral vector production and quantification methods -- Baculovirus expression vector system (BEVS) : From proteins to virus-like particles and viral vectors -- Production of adeno-associated viral vectors in insect cells using triple infection : optimization of baculovirus concentration ratios -- Complementing results : additional evaluation of the multiplicity of infection -- Quadruple infection : use of two monocistronic bacreps to supply aav rep genes -- Improving aav vector yield in insect cells by modulating the temperature after infection -- A study on how to increase the cell density at infection without decreasing the specific production of AAV vectors.

Uncontrolled Keywords

Génie génétique microbien; Thérapie génique; Baculovirus recombinants

Open Access document in PolyPublie
Additional Information: Le fichier PDF de ce document a été produit par Bibliothèque et Archives Canada selon les termes du programme Thèses Canada https://canada.on.worldcat.org/oclc/428866627
Department: Département de génie chimique
Date Deposited: 04 Aug 2021 11:04
Last Modified: 25 Aug 2021 14:58
PolyPublie URL: https://publications.polymtl.ca/8052/

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