Fiona Milano, Anik Chevrier, Gregory De Crescenzo et Marc Lavertu
Article de revue (2023)
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Résumé
Freeze-dried chitosan formulations solubilized in platelet-rich plasma (PRP) are currently evaluated as injectable implants with the potential for augmenting the standard of care for tissue repair in different orthopedic conditions. The present study aimed to shorten the solidification time of such implants, leading to an easier application and a facilitated solidification in a wet environment, which were direct demands from orthopedic surgeons. The addition of thrombin to the formulation before lyophilization was explored. The challenge was to find a formulation that coagulated fast enough to be applied in a wet environment but not too fast, which would make handling/injection difficult. Four thrombin concentrations were analyzed (0.0, 0.25, 0.5, and 1.0 NIH/mL) in vitro (using thromboelastography, rheology, indentation, syringe injectability, and thrombin activity tests) as well as ex vivo (by assessing the implant’s adherence to tendon tissue in a wet environment). The biomaterial containing 0.5 NIH/mL of thrombin significantly increased the coagulation speed while being easy to handle up to 6 min after solubilization. Furthermore, the adherence of the biomaterial to tendon tissues was impacted by the biomaterial-tendon contact duration and increased faster when thrombin was present. These results suggest that our biomaterial has great potential for use in regenerative medicine applications.
Mots clés
chitosan; thrombin; platelet-rich plasma; injectable implant; tissue regeneration; solidification properties
Sujet(s): |
1800 Génie chimique > 1800 Génie chimique 1900 Génie biomédical > 1900 Génie biomédical |
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Département: |
Département de génie chimique Institut de génie biomédical |
Organismes subventionnaires: | Fonds de recherche du Québec – Nature et technologies, ChitogenX, Natural Sciences and Engineering Research Council of Canad |
Numéro de subvention: | 304445, 11957-2018 |
URL de PolyPublie: | https://publications.polymtl.ca/54360/ |
Titre de la revue: | Polymers (vol. 15, no 13) |
Maison d'édition: | Multidisciplinary Digital Publishing Institute |
DOI: | 10.3390/polym15132919 |
URL officielle: | https://doi.org/10.3390/polym15132919 |
Date du dépôt: | 15 sept. 2023 15:45 |
Dernière modification: | 05 avr. 2024 14:04 |
Citer en APA 7: | Milano, F., Chevrier, A., De Crescenzo, G., & Lavertu, M. (2023). Injectable Lyophilized Chitosan-Thrombin-Platelet-Rich Plasma (CS-FIIa-PRP) Implant to Promote Tissue Regeneration: In Vitro and Ex Vivo Solidification Properties. Polymers, 15(13), 2919 (15 pages). https://doi.org/10.3390/polym15132919 |
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