Pablo A. Valdés, Frédéric Leblond, Valerie L. Jacobs, Brian C. Wilson, Keith D. Paulsen et David W. Roberts
Article de revue (2012)
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Abstract
Intraoperative visual fluorescence imaging (vFI) has emerged as a promising aid to surgical guidance, but does not fully exploit the potential of the fluorescent agents that are currently available. Here, we introduce a quantitative fluorescence imaging (qFI) approach that converts spectrally-resolved data into images of absolute fluorophore concentration pixel-by-pixel across the surgical field of view (FOV). The resulting estimates are linear, accurate, and precise relative to true values, and spectral decomposition of multiple fluorophores is also achieved. Experiments with protoporphyrin IX in a glioma rodent model demonstrate in vivo quantitative and spectrally-resolved fluorescence imaging of infiltrating tumor margins for the first time. Moreover, we present images from human surgery which detect residual tumor not evident with state-of-the-art vFI. The wide-field qFI technique has broad implications for intraoperative surgical guidance because it provides near real-time quantitative assessment of multiple fluorescent biomarkers across the operative field.
Mots clés
Animals; Brain Neoplasms/diagnosis/*surgery; *Fluorescence; Fluorescent Dyes; Glioma/diagnosis/surgery; Humans; Intraoperative Period; Optical Imaging/instrumentation/*methods; Phantoms, Imaging; Protoporphyrins; Rats
Sujet(s): |
3100 Physique > 3100 Physique 3100 Physique > 3108 Méthodes expérimentales et instrumentation |
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Département: | Département de génie physique |
Organismes subventionnaires: | National Institutes of Health, Hitchcock Foundation Grant (PAV), Norris Cotton Cancer Multiple Investigator Prouty Grant (KDP, DWR, PAV) |
Numéro de subvention: | R01NS052274-01A2 (DWR), K25CA138578 (FL) |
URL de PolyPublie: | https://publications.polymtl.ca/5158/ |
Titre de la revue: | Scientific Reports (vol. 2, no 1) |
Maison d'édition: | Nature |
DOI: | 10.1038/srep00798 |
URL officielle: | https://doi.org/10.1038/srep00798 |
Date du dépôt: | 05 avr. 2022 15:23 |
Dernière modification: | 05 avr. 2024 23:39 |
Citer en APA 7: | Valdés, P. A., Leblond, F., Jacobs, V. L., Wilson, B. C., Paulsen, K. D., & Roberts, D. W. (2012). Quantitative, spectrally-resolved intraoperative fluorescence imaging. Scientific Reports, 2(1), 798. https://doi.org/10.1038/srep00798 |
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