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Quantitative, spectrally-resolved intraoperative fluorescence imaging

P. A. Valdes, Frédéric Leblond, V. L. Jacobs, B. C. Wilson, K. D. Paulsen and D. W. Roberts

Article (2012)

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Intraoperative visual fluorescence imaging (vFI) has emerged as a promising aid to surgical guidance, but does not fully exploit the potential of the fluorescent agents that are currently available. Here, we introduce a quantitative fluorescence imaging (qFI) approach that converts spectrally-resolved data into images of absolute fluorophore concentration pixel-by-pixel across the surgical field of view (FOV). The resulting estimates are linear, accurate, and precise relative to true values, and spectral decomposition of multiple fluorophores is also achieved. Experiments with protoporphyrin IX in a glioma rodent model demonstrate in vivo quantitative and spectrally-resolved fluorescence imaging of infiltrating tumor margins for the first time. Moreover, we present images from human surgery which detect residual tumor not evident with state-of-the-art vFI. The wide-field qFI technique has broad implications for intraoperative surgical guidance because it provides near real-time quantitative assessment of multiple fluorescent biomarkers across the operative field.

Uncontrolled Keywords

Animals; Brain Neoplasms/diagnosis/*surgery; *Fluorescence; Fluorescent Dyes; Glioma/diagnosis/surgery; Humans; Intraoperative Period; Optical Imaging/instrumentation/*methods; Phantoms, Imaging; Protoporphyrins; Rats

Subjects: 3100 Physics > 3100 Physics
3100 Physics > 3108 Experimental methods and instrumentation
Department: Department of Engineering Physics
Funders: National Institutes of Health, Hitchcock Foundation Grant (PAV), Norris Cotton Cancer Multiple Investigator Prouty Grant (KDP, DWR, PAV)
Grant number: R01NS052274-01A2 (DWR), K25CA138578 (FL)
PolyPublie URL: https://publications.polymtl.ca/5158/
Journal Title: Scientific Reports (vol. 2, no. 1)
Publisher: Nature
DOI: 10.1038/srep00798
Official URL: https://doi.org/10.1038/srep00798
Date Deposited: 05 Apr 2022 15:23
Last Modified: 30 Sep 2023 12:53
Cite in APA 7: Valdes, P. A., Leblond, F., Jacobs, V. L., Wilson, B. C., Paulsen, K. D., & Roberts, D. W. (2012). Quantitative, spectrally-resolved intraoperative fluorescence imaging. Scientific Reports, 2(1), 798. https://doi.org/10.1038/srep00798


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