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Hospital drains as reservoirs of Pseudomonas aeruginosa: Multiple-locus variable-number of tandem repeats analysis genotypes recovered from faucets, sink surfaces and patients

Cindy Lalancette, Dominique Charron, Céline Laferriere, Patrick Dolce, Éric Déziel, Michèle Prévost and Émilie Bédard

Article (2017)

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Cite this document: Lalancette, C., Charron, D., Laferriere, C., Dolce, P., Déziel, É., Prévost, M. & Bédard, É. (2017). Hospital drains as reservoirs of Pseudomonas aeruginosa: Multiple-locus variable-number of tandem repeats analysis genotypes recovered from faucets, sink surfaces and patients. Pathogens, 6(3). doi:10.3390/pathogens6030036
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Abstract

Identifying environmental sources of Pseudomonas aeruginosa (Pa) related to hospital-acquired infections represents a key challenge for public health. Biofilms in water systems offer protection and favorable growth conditions, and are prime reservoirs of microorganisms. A comparative genotyping survey assessing the relationship between Pa strains recovered in hospital sink biofilm and isolated in clinical specimens was conducted. Environmental strains from drain, faucet and sink-surface biofilm were recovered by a culture method after an incubation time ranging from 48 to 240 h. The genotyping of 38 environmental and 32 clinical isolates was performed using a multiple-locus variable-number of tandem repeats analysis (MLVA). More than one-third of Pa isolates were only cultivable following >/=48 h of incubation, and were predominantly from faucet and sink-surface biofilms. In total, 41/70 strains were grouped within eight genotypes (A to H). Genotype B grouped a clinical and an environmental strain isolated in the same ward, 5 months apart, suggesting this genotype could thrive in both contexts. Genotype E grouped environmental isolates that were highly prevalent throughout the hospital and that required a longer incubation time. The results from the multi-hospital follow-up study support the drain as an important reservoir of Pa dissemination to faucets, sink surfaces and patients. Optimizing the recovery of environmental strains will strengthen epidemiological investigations, facilitate pathway identification, and assist in identifying and controlling the reservoirs potentially associated to hospital-acquired infections.

Uncontrolled Keywords

Mlva; Pseudomonas aeruginosa; environmental reservoir; genotyping; heathcare-acquired infections; sink environment

Open Access document in PolyPublie
Subjects: 1000 Génie civil > 1000 Génie civil
1500 Génie de l'environnement > 1500 Génie de l'environnement
1500 Génie de l'environnement > 1501 Qualité de l'eau, pollution
Department: Département des génies civil, géologique et des mines
Research Center: Autre
Funders: NSERC Industrial Chair on Drinking Water / Chaire industrielle CRSNG en eau potable
Date Deposited: 17 Dec 2021 10:47
Last Modified: 18 Dec 2021 01:20
PolyPublie URL: https://publications.polymtl.ca/4986/
Document issued by the official publisher
Journal Title: Pathogens (vol. 6, no. 3)
Publisher: MDPI
Official URL: https://doi.org/10.3390/pathogens6030036

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