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Identification of factors affecting bacterial abundance and community structures in a full-scale chlorinated drinking water distribution system

Vanessa C. F. Dias, Audrey-Anne Durand, Philippe Constant, Michèle Prévost and Emilie Bédard

Article (2019)

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Cite this document: Dias, V. C. F., Durand, A.-A., Constant, P., Prévost, M. & Bédard, E. (2019). Identification of factors affecting bacterial abundance and community structures in a full-scale chlorinated drinking water distribution system. Water, 11(3). doi:10.3390/w11030627
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Abstract

Disentangling factors influencing suspended bacterial community structure across distribution system and building plumbing provides insight into microbial control strategies from source to tap. Water quality parameters (residence time, chlorine, and total cells) and bacterial community structure were investigated across a full-scale chlorinated drinking water distribution system. Sampling was conducted in treated water, in different areas of the distribution system and in hospital building plumbing. Bacterial community was evaluated using 16S rRNA gene sequencing. Bacterial community structure clearly differed between treated, distributed, and premise plumbing water samples. While Proteobacteria (60%), Planctomycetes (20%), and Bacteroidetes (10%) were the most abundant phyla in treated water, Proteobacteria largely dominated distribution system sites (98%) and taps (91%). Distributed and tap water differed in their Proteobacteria profile: Alphaproteobacteria was dominant in distributed water (92% vs. 65% in tap waters), whereas Betaproteobacteria was most abundant in tap water (18% vs. 2% in the distribution system). Finally, clustering of bacterial community profiles was largely explained by differences in chlorine residual concentration, total bacterial count, and water residence time. Residual disinfectant and hydraulic residence time were determinant factors of the community structure in main pipes and building plumbing, rather than treated water bacterial communities.

Uncontrolled Keywords

drinking water distribution system; chlorine residual; residence time; high-throughput sequencing; building plumbing; bacterial community structure

Open Access document in PolyPublie
Subjects: 1000 Génie civil > 1000 Génie civil
1000 Génie civil > 1007 Ressources et approvisionnement en eau
5200 Microbiologie > 5201 Bactériologie
Department: Département des génies civil, géologique et des mines
Research Center: Non applicable
Funders: CRSNG/NSERC Industrial Chair on Drinking Water
Date Deposited: 17 Mar 2021 11:10
Last Modified: 08 Apr 2021 10:42
PolyPublie URL: https://publications.polymtl.ca/4845/
Document issued by the official publisher
Journal Title: Water (vol. 11, no. 3)
Publisher: MDPI
Official URL: https://doi.org/10.3390/w11030627

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