Mounia Arkoun, France Daigle, Marie-Claude Heuzey and Abdellah Ajji
Article (2017)
|
Published Version Terms of Use: Creative Commons Attribution . Download (4MB) |
Cite this document: | Arkoun, M., Daigle, F., Heuzey, M.-C. & Ajji, A. (2017). Antibacterial electrospun chitosan-based nanofibers: A bacterial membrane perforator. Food Science & Nutrition, 5(4), p. 865-874. doi:10.1002/fsn3.468 |
---|
Abstract
This study investigates the antibacterial action of chitosan-based nanofibers (CNFs) obtained by the electrospinning process on the permeability of bacterial membranes. The bactericidal efficiency of CNFs was first determined against Gram-negative Escherichia coli and Salmonella Typhimurium, and Gram-positive Staphylococcus aureus and Listeria innocua bacteria as a baseline. The results strongly suggest that CNFs interact with the negatively charged bacterial cell wall causing membrane rupture and inducing leakage of intracellular components among which are proteins and DNA. Results clearly indicate that the release of such components after contact with CNFs is an indication of membrane permeabilization and perforation, as pore formation was observed in transmission electron microscopy (TEM). This work suggests a plausible antibacterial mechanism of action of CNFs and also provides clear evidence in favor of chitosan as a bacterial membrane disruptor and perforator. As a result, CNFs can find promising applications as bioactive food packaging materials capable to extend shelf life of food products while inhibiting the spread of alteration flora and foodborne pathogens.
Uncontrolled Keywords
antibacterial; electrospun chitosan-based nanofibers; membrane perforation; membrane permeability
![]() |
|
Subjects: |
1800 Génie chimique > 1800 Génie chimique 2000 Science et technologie des matériaux > 2004 Polymères et revêtements 5200 Microbiologie > 5201 Bactériologie |
---|---|
Department: | Département de génie chimique |
Research Center: | CREPEC - Centre de recherche sur les systèmes polymères et composites à haute performance |
Funders: | Fonds de Recherche du Québec ‐ Nature et Technologies (FRQNT), CRSNG/NSERC |
Date Deposited: | 11 Mar 2021 12:07 |
Last Modified: | 08 Apr 2021 10:43 |
PolyPublie URL: | https://publications.polymtl.ca/4753/ |
![]() |
|
Journal Title: | Food Science & Nutrition (vol. 5, no. 4) |
---|---|
Publisher: | Wiley |
Official URL: | https://doi.org/10.1002/fsn3.468 |
Statistics
Total downloads
Downloads per month in the last year
Origin of downloads
Dimensions