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Antibacterial electrospun chitosan-based nanofibers: A bacterial membrane perforator

Mounia Arkoun, France Daigle, Marie-Claude Heuzey and Abdellah Ajji

Article (2017)

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Abstract

This study investigates the antibacterial action of chitosan-based nanofibers (CNFs) obtained by the electrospinning process on the permeability of bacterial membranes. The bactericidal efficiency of CNFs was first determined against Gram-negative Escherichia coli and Salmonella Typhimurium, and Gram-positive Staphylococcus aureus and Listeria innocua bacteria as a baseline. The results strongly suggest that CNFs interact with the negatively charged bacterial cell wall causing membrane rupture and inducing leakage of intracellular components among which are proteins and DNA. Results clearly indicate that the release of such components after contact with CNFs is an indication of membrane permeabilization and perforation, as pore formation was observed in transmission electron microscopy (TEM). This work suggests a plausible antibacterial mechanism of action of CNFs and also provides clear evidence in favor of chitosan as a bacterial membrane disruptor and perforator. As a result, CNFs can find promising applications as bioactive food packaging materials capable to extend shelf life of food products while inhibiting the spread of alteration flora and foodborne pathogens.

Uncontrolled Keywords

antibacterial; electrospun chitosan-based nanofibers; membrane perforation; membrane permeability

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
2000 Materials science and technology > 2004 Polymers and coatings
5200 Microbiology > 5201 Bacteriology
Department: Department of Chemical Engineering
Research Center: CREPEC - Center for Applied Research on Polymers and Composites
Funders: Fonds de Recherche du Québec ‐ Nature et Technologies (FRQNT), CRSNG/NSERC
PolyPublie URL: https://publications.polymtl.ca/4753/
Journal Title: Food Science & Nutrition (vol. 5, no. 4)
Publisher: Wiley
DOI: 10.1002/fsn3.468
Official URL: https://doi.org/10.1002/fsn3.468
Date Deposited: 11 Mar 2021 12:07
Last Modified: 09 Apr 2024 17:33
Cite in APA 7: Arkoun, M., Daigle, F., Heuzey, M.-C., & Ajji, A. (2017). Antibacterial electrospun chitosan-based nanofibers: A bacterial membrane perforator. Food Science & Nutrition, 5(4), 865-874. https://doi.org/10.1002/fsn3.468

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