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Antibacterial electrospun chitosan-based nanofibers: A bacterial membrane perforator

Mounia Arkoun, France Daigle, Marie-Claude Heuzey and Abdellah Ajji

Article (2017)

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Cite this document: Arkoun, M., Daigle, F., Heuzey, M.-C. & Ajji, A. (2017). Antibacterial electrospun chitosan-based nanofibers: A bacterial membrane perforator. Food Science & Nutrition, 5(4), p. 865-874. doi:10.1002/fsn3.468
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Abstract

This study investigates the antibacterial action of chitosan-based nanofibers (CNFs) obtained by the electrospinning process on the permeability of bacterial membranes. The bactericidal efficiency of CNFs was first determined against Gram-negative Escherichia coli and Salmonella Typhimurium, and Gram-positive Staphylococcus aureus and Listeria innocua bacteria as a baseline. The results strongly suggest that CNFs interact with the negatively charged bacterial cell wall causing membrane rupture and inducing leakage of intracellular components among which are proteins and DNA. Results clearly indicate that the release of such components after contact with CNFs is an indication of membrane permeabilization and perforation, as pore formation was observed in transmission electron microscopy (TEM). This work suggests a plausible antibacterial mechanism of action of CNFs and also provides clear evidence in favor of chitosan as a bacterial membrane disruptor and perforator. As a result, CNFs can find promising applications as bioactive food packaging materials capable to extend shelf life of food products while inhibiting the spread of alteration flora and foodborne pathogens.

Uncontrolled Keywords

antibacterial; electrospun chitosan-based nanofibers; membrane perforation; membrane permeability

Open Access document in PolyPublie
Subjects: 1800 Génie chimique > 1800 Génie chimique
2000 Science et technologie des matériaux > 2004 Polymères et revêtements
5200 Microbiologie > 5201 Bactériologie
Department: Département de génie chimique
Research Center: CREPEC - Centre de recherche sur les systèmes polymères et composites à haute performance
Funders: Fonds de Recherche du Québec ‐ Nature et Technologies (FRQNT), CRSNG/NSERC
Date Deposited: 11 Mar 2021 12:07
Last Modified: 08 Apr 2021 10:43
PolyPublie URL: https://publications.polymtl.ca/4753/
Document issued by the official publisher
Journal Title: Food Science & Nutrition (vol. 5, no. 4)
Publisher: Wiley
Official URL: https://doi.org/10.1002/fsn3.468

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