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Long-term fluorescence hyperspectral imaging of on-chip treated co-culture tumour spheroids to follow clonal evolution

Amélie St-Georges-Robillard, Maxime Cahuzac, Benjamin Péant, Hubert Fleury, Muhammad Abdul Lateef, Alexis Ricard, Alexandre Sauriol, Frédéric Leblond, Anne-Marie Mes-Masson and Thomas Gervais

Article (2019)

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Abstract

Multicellular tumour spheroids are an ideal in vitro tumour model to study clonal heterogeneity and drug resistance in cancer research because different cell types can be mixed at will. However, measuring the individual response of each cell population over time is challenging: current methods are either destructive, such as flow cytometry, or cannot image throughout a spheroid, such as confocal microscopy. Our group previously developed a wide-field fluorescence hyperspectral imaging system to study spheroids formed and cultured in microfluidic chips. In the present study, two subclones of a single parental ovarian cancer cell line transfected to express different fluorophores were produced and co-culture spheroids were formed on-chip using ratios forming highly asymmetric subpopulations. We performed a 3D proliferation assay on each cell population forming the spheroids that matched the 2D growth behaviour. Response assays to PARP inhibitors and platinum-based drugs were also performed to follow the clonal evolution of mixed populations. Our experiments show that hyperspectral imaging can detect spheroid response before observing a decrease in spheroid diameter. Hyperspectral imaging and microfluidic-based spheroid assays provide a versatile solution to study clonal heterogeneity, able to measure response in subpopulations presenting as little as 10% of the initial spheroid.

Uncontrolled Keywords

hyperspectral imaging; spheroids; microfluidics; cancer; clonal populations

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
1900 Biomedical engineering > 1901 Biomedical technology
3100 Physics > 3101 Atomic and molecular studies
Department: Department of Engineering Physics
Institut de génie biomédical
Funders: NSERC / CRSNG, Fonds de recherche du Québec – Nature et technologies, Cancer Research Society, Ovarian Cancer Canada, Canada Foundation for Innovation, CMC Microsystems, TransMedTech Institute, Canada First Research Excellence Fund
Grant number: 20103
PolyPublie URL: https://publications.polymtl.ca/44065/
Journal Title: Integrative Biology (vol. 11, no. 4)
Publisher: Oxford Academic
DOI: 10.1093/intbio/zyz012
Official URL: https://doi.org/10.1093/intbio/zyz012
Date Deposited: 18 Apr 2023 15:02
Last Modified: 22 Apr 2024 09:14
Cite in APA 7: St-Georges-Robillard, A., Cahuzac, M., Péant, B., Fleury, H., Lateef, M. A., Ricard, A., Sauriol, A., Leblond, F., Mes-Masson, A.-M., & Gervais, T. (2019). Long-term fluorescence hyperspectral imaging of on-chip treated co-culture tumour spheroids to follow clonal evolution. Integrative Biology, 11(4), 130-141. https://doi.org/10.1093/intbio/zyz012

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