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Phenols removal from hemicelluloses pre-hydrolysate by laccase to improve butanol production

Rosalie Allard-Massicotte, Hassan Chadjaa and Mariya Marinova

Article (2017)

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Cite this document: Allard-Massicotte, R., Chadjaa, H. & Marinova, M. (2017). Phenols removal from hemicelluloses pre-hydrolysate by laccase to improve butanol production. Fermentation, 3(3). doi:10.3390/fermentation3030031
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Phenolic compounds are important inhibitors of the microorganisms used in the Acetone-Butanol-Ethanol (ABE) fermentation. The degradation of phenolic compounds in a wood pre-hydrolysate, a potential substrate for the production of ABE, was studied in this article. First, physicochemical methods for detoxification such as nanofiltration and flocculation were applied and the best combination was selected. With a flocculated sample, the concentration of phenolic compounds decreases from 1.20 to 0.28 g/L with the addition of a solid laccase at optimum conditions, which is below the phenolic compounds limit of inhibition. This results in an increase in butanol production, more than double, compared to a pre-hydrolysate non-treated with laccase enzymes.

Uncontrolled Keywords

phenolic compounds; detoxification; butanol; pre-hydrolysate; laccase enzymes

Open Access document in PolyPublie
Subjects: 1800 Génie chimique > 1800 Génie chimique
2300 Combustibles et technologie de l'énergie > 2303 Autres sources d'énergie (combustibles solaire, éolien, etc.)
5400 Biochimie > 5400 Biochimie
Department: Département de génie chimique
Research Center: Non applicable
Funders: CRSNG/NSERC, BioFuelNet Canada
Grant number: 437803-12
Date Deposited: 09 Mar 2020 14:06
Last Modified: 08 Apr 2021 10:43
PolyPublie URL: https://publications.polymtl.ca/4218/
Document issued by the official publisher
Journal Title: Fermentation (vol. 3, no. 3)
Publisher: MDPI
Official URL: https://doi.org/10.3390/fermentation3030031


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