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Optimization of lipids production by Cryptococcus laurentii 11 using cheese whey with molasses

Rodrigo Fernandes Castanha, Adriano Pinto Mariano, Lilia Aparecida Salgado de Morais, Shirlei Scramin, Regina Teresa Rosim Monteiro

Article (2014)

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Abstract

This study aimed the optimization of culture condition and composition for production ofCryptococcus laurentii 11 biomass and lipids in cheese whey medium supplemented with sugarcanemolasses. The optimization of pH, fermentation time, and molasses concentration according to a fullfactorial statistical experimental design was followed by a Plackett-Burman experimental design,which was used to determine whether the supplementation of the culture medium by yeast extract andinorganic salts could provide a further enhancement of lipids production. The following conditionsand composition of the culture medium were found to optimize biomass and lipids production: 360 hfermentation, 6.5 pH and supplementation of (g L-1): 50 molasses, 0.5 yeast extract, 4 KH2PO4, 1Na2HPO4, 0.75 MgSO4•7H2O and 0.002 ZnSO4•H2O. Additional supplementation with inorganicsalts and yeast extract was essential to optimize the production, in terms of product concentration andproductivity, of neutral lipids by C. laurentii 11. Under this optimized condition, the production of totallipids increased by 133% in relation to control experiment (from 1.27 to 2.96 g L-1). The totallipids indicated a predominant (86%) presence of neutral lipids with high content of 16- and 18- carbon-chain saturated and monosaturated fatty acids. This class of lipids is considered especially suitablefor the production of biodiesel.

Uncontrolled Keywords

Cryptococcus laurentii, microbial lipids, cheese whey, sugarcane molasses, biodiesel.

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
5200 Microbiology > 5201 Bacteriology
5400 Biochemistry > 5405 Lipids
Department: Department of Chemical Engineering
Funders: Jamava Laticínios, Fermentec, Algae Biotechnology (Brazil), National Council for Scientific and Technological Development (CNPq)
PolyPublie URL: https://publications.polymtl.ca/3624/
Journal Title: Brazilian Journal of Microbiology (vol. 45, no. 2)
Publisher: Sociedade Brasileira de Microbiologia
DOI: 10.1590/s1517-83822014000200003
Official URL: https://doi.org/10.1590/s1517-83822014000200003
Date Deposited: 17 Feb 2020 11:54
Last Modified: 11 Nov 2022 14:07
Cite in APA 7: Castanha, R. F., Mariano, A. P., Morais, L. A. S. , Scramin, S., & Monteiro, R. T. R. (2014). Optimization of lipids production by Cryptococcus laurentii 11 using cheese whey with molasses. Brazilian Journal of Microbiology, 45(2), 379-387. https://doi.org/10.1590/s1517-83822014000200003

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