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Combination of heat shock and enhanced thermal regime to control the growth of a persistent Legionella pneumophila strain

Émilie Bédard, Inès Boppe, Serge Kouame, Philippe Martin, Linda Pinsonneault, Louis Valiquette, Jules Racine and Michèle Prévost

Article (2016)

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Cite this document: Bédard, É., Boppe, I., Kouame, S., Martin, P., Pinsonneault, L., Valiquette, L., ... Prévost, M. (2016). Combination of heat shock and enhanced thermal regime to control the growth of a persistent Legionella pneumophila strain. Pathogens, 5(2). doi:10.3390/pathogens5020035
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Abstract

Following nosocomial cases of Legionella pneumophila, the investigation of a hot water system revealed that 81.5% of sampled taps were positive for L. pneumophila, despite the presence of protective levels of copper in the water. A significant reduction of L. pneumophila counts was observed by culture after heat shock disinfection. The following corrective measures were implemented to control L. pneumophila: increasing the hot water temperature (55 to 60 degrees C), flushing taps weekly with hot water, removing excess lengths of piping and maintaining a water temperature of 55 degrees C throughout the system. A gradual reduction in L. pneumophila counts was observed using the culture method and qPCR in the 18 months after implementation of the corrective measures. However, low level contamination was retained in areas with hydraulic deficiencies, highlighting the importance of maintaining a good thermal regime at all points within the system to control the population of L. pneumophila.

Uncontrolled Keywords

Legionella pneumophila; copper concentration; culture; hospital premise plumbing; legionellosis; quantitative polymerase chain reaction; sanitary hot water; temperature diagnostic

Open Access document in PolyPublie
Subjects: 1000 Génie civil > 1007 Ressources et approvisionnement en eau
5200 Microbiologie > 5201 Bactériologie
Department: Département des génies civil, géologique et des mines
Research Center: Non applicable
Funders: CRSNG / NSERC Industrial Chair on Drinking Water
Date Deposited: 10 Mar 2020 14:45
Last Modified: 11 Mar 2020 01:20
PolyPublie URL: https://publications.polymtl.ca/3603/
Document issued by the official publisher
Journal Title: Pathogens (vol. 5, no. 2)
Publisher: MDPI
Official URL: https://doi.org/10.3390/pathogens5020035

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