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Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons

Flavie Lavoie-Cardinal, Charleen Salesse, Éric Bergeron, Michel Meunier and Paul De Koninck

Article (2016)

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Cite this document: Lavoie-Cardinal, F., Salesse, C., Bergeron, É., Meunier, M. & De Koninck, P. (2016). Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons. Scientific Reports, 6, p. 1-9. doi:10.1038/srep20619
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Abstract

Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investigate localized signaling has been limited. Here, we introduce an all optical method for the stimulation and the monitoring of localized Ca2+ signaling in neurons that takes advantage of plasmonic excitation of gold nanoparticles (AuNPs). We show with confocal microscopy that 800 nm laser pulse application onto a neuron decorated with a few AuNPs triggers a transient increase in free Ca2+, measured optically with GCaMP6s. We show that action potentials, measured electrophysiologically, can be induced with this approach. We demonstrate activation of local Ca2+ transients and Ca2+ signaling via CaMKII in dendritic domains, by illuminating a single or few functionalized AuNPs specifically targeting genetically-modified neurons. This NP-Assisted Localized Optical Stimulation (NALOS) provides a new complement to light-dependent methods for controlling neuronal activity and cell signaling.

Uncontrolled Keywords

Neurons; Membranes; Cells; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Gold; Complement System Proteins; Action Potentials; Light; Lasers; Microscopy, Confocal; Biophysical methods; Ca2+ imaging; Fluorescence imaging; Nanoparticles; Nanoscale biophysics

Open Access document in PolyPublie
Subjects: 1900 Génie biomédical > 1900 Génie biomédical
1900 Génie biomédical > 1902 Matériaux biomédicaux
3100 Physique > 3100 Physique
3300 Physique de la matière condensée > 3306 Propriétés optiques
Department: Département de génie physique
Research Center: Autre
Funders: Chaires de recherche du Canada, CRSNG/NSERC, Fonds de recherche Québec--Santé
Date Deposited: 06 Dec 2018 13:05
Last Modified: 07 Dec 2018 01:20
PolyPublie URL: https://publications.polymtl.ca/3523/
Document issued by the official publisher
Journal Title: Scientific Reports (vol. 6)
Publisher: Nature Research
Official URL: https://doi.org/10.1038/srep20619

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