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A quantitative metabolomics study of high sodium response in Clostridium acetobutylicum ATCC 824 acetone-butanol-ethanol (ABE) fermentation

Xinhe Zhao, Stefan Condruz, Jingkui Chen and Mario Jolicoeur

Article (2016)

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Cite this document: Zhao, X., Condruz, S., Chen, J. & Jolicoeur, M. (2016). A quantitative metabolomics study of high sodium response in Clostridium acetobutylicum ATCC 824 acetone-butanol-ethanol (ABE) fermentation. Scientific Reports, 6, p. 1-13. doi:10.1038/srep28307
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Hemicellulose hydrolysates, sugar-rich feedstocks used in biobutanol refinery, are normally obtained by adding sodium hydroxide in the hydrolyze process. However, the resulting high sodium concentration in the hydrolysate inhibits ABE (acetone-butanol-ethanol) fermentation, and thus limits the use of these low-cost feedstocks. We have thus studied the effect of high sodium on the metabolic behavior of Clostridium acetobutyricum ATCC 824, with xylose as the carbon source. At a threshold sodium concentration of 200 mM, a decrease of the maximum cell dry weight (-19.50 +/- 0.85%) and of ABE yield (-35.14 +/- 3.50% acetone, -33.37 +/- 0.74% butanol, -22.95 +/- 1.81% ethanol) were observed compared to control culture. However, solvents specific productivities were not affected by supplementing sodium. The main effects of high sodium on cell metabolism were observed in acidogenesis, during which we observed the accumulation of ATP and NADH, and the inhibition of the pentose phosphate (PPP) and the glycolytic pathways with up to 80.73 +/- 1.47% and 68.84 +/- 3.42% decrease of the associated metabolic intermediates, respectively. However, the NADP+-to-NADPH ratio was constant for the whole culture duration, a phenomenon explaining the robustness of solvents specific productivities. Therefore, high sodium, which inhibited biomass growth through coordinated metabolic effects, interestingly triggered cell robustness on solvents specific productivity.

Open Access document in PolyPublie
Subjects: 1800 Génie chimique > 1800 Génie chimique
1800 Génie chimique > 1802 Génie biochimique
Department: Département de génie chimique
Research Center: Autre
Funders: BioFuelNet Center of Excellence, CRSNG/NSERC
Date Deposited: 06 Dec 2018 12:44
Last Modified: 08 Apr 2021 10:43
PolyPublie URL: https://publications.polymtl.ca/3519/
Document issued by the official publisher
Journal Title: Scientific Reports (vol. 6)
Publisher: Nature Research
Official URL: https://doi.org/10.1038/srep28307


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