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A Facile Approach for the Mass Production of Submicro/Micro Poly (Lactic Acid) Fibrous Mats and Their Cytotoxicity Test towards Neural Stem Cells

Afra Hadjizadeh, Houman Savoji and Abdellah Ajji

Article (2016)

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Terms of Use: Creative Commons Attribution.
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Cite this document: Hadjizadeh, A., Savoji, H. & Ajji, A. (2016). A Facile Approach for the Mass Production of Submicro/Micro Poly (Lactic Acid) Fibrous Mats and Their Cytotoxicity Test towards Neural Stem Cells. BioMed Research International, 2016. doi:10.1155/2016/8921316
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Despite many of the studies being conducted, the electrospinning of poly (lactic acid) (PLA), dissolved in its common solvents, is difficult to be continuously processed for mass production. This is due to the polymer solution droplet drying. Besides, the poor stretching capability of the polymer solution limits the production of small diameter fibers. To address these issues, we have examined the two following objectives: first, using an appropriate solvent system for the mass production of fibrousmats with fine tunable fiber diameters; second, nontoxicity of the mats towards Neural Stem Cell (NSC). To this aim, TFA(trifluoroacetic acid) was used as a cosolvent, in a mixture with DCM(dichloromethane), and the solution viscosity, surface tension, electrical conductivity, and the continuity of the electrospinning process were compared with the solutions prepared with common single solvents. The binary solvent facilitated PLA electrospinning, resulting in a long lasting, stable electrospinning condition, due to the low surface tension and high conductivity of the binary-solvent system. The fiber diameter was tailored from nano to micro by varying effective parameters and examined by scanning electron microscopy (SEM) and image-processing software. Laminin-coated electrospun mats supported NSC expansion and spreading, as examined using AlamarBlue assay and fluorescent microscopy, respectively.

Uncontrolled Keywords

Cell Line; Cell Survival; Humans; Materials Testing; Neural Stem Cells; Polyesters; Porosity; Polyesters; poly(lactide)

Open Access document in PolyPublie
Subjects: 1800 Génie chimique > 1800 Génie chimique
Department: Département de génie chimique
Research Center: CREPEC - Centre de recherche sur les systèmes polymères et composites à haute performance
Funders: CRSNG / NSERC
Date Deposited: 25 Oct 2018 16:14
Last Modified: 26 Oct 2018 01:20
PolyPublie URL: https://publications.polymtl.ca/3495/
Document issued by the official publisher
Journal Title: BioMed Research International (vol. 2016)
Publisher: Hindawi Publishing Corporation
Official URL: https://doi.org/10.1155/2016/8921316


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