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Two-Aperture Microfluidic Probes as Flow Dipoles: Theory and Applications

Mohammadali Safavieh, Mohammad A. Qasaimeh, Ali Vakil, David Juncker and Thomas Gervais

Article (2015)

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Cite this document: Safavieh, M., Qasaimeh, M. A., Vakil, A., Juncker, D. & Gervais, T. (2015). Two-Aperture Microfluidic Probes as Flow Dipoles: Theory and Applications. Scientific Reports, 5. doi:10.1038/srep11943
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Abstract

A microfluidic probe (MFP) is a mobile channel-less microfluidic system under which a fluid is injected from an aperture into an open space, hydrodynamically confined by a surrounding fluid, and entirely re-aspirated into a second aperture. Various MFPs have been developed, and have been used for applications ranging from surface patterning of photoresists to local perfusion of organotypic tissue slices. However, the hydrodynamic and mass transfer properties of the flow under the MFP have not been analyzed, and the flow parameters are adjusted empirically. Here, we present an analytical model describing the key transport properties in MFP operation, including the dimensions of the hydrodynamic flow confinement (HFC) area, diffusion broadening, and shear stress as a function of: (i) probe geometry (ii) aspiration-to-injection flow rate ratio (iii) gap between MFP and substrate and (iv) reagent diffusivity. Analytical results and scaling laws were validated against numerical simulations and experimental results from published data. These results will be useful to guide future MFP design and operation, notably to control the MFP "brush stroke" while preserving shear-sensitive cells and tissues.

Uncontrolled Keywords

Algorithms; Microfluidic Analytical Techniques; Microfluidics; Models, Theoretical

Open Access document in PolyPublie
Subjects: 2700 Technologie de l'information > 2713 Algorithmes
3100 Physique > 3100 Physique
Department: Département de génie physique
Research Center: Autre
Funders: Fonds québécois de la recherche sur la nature et les technologies (FQRNT), CRSNG / NSERC, ISS / NSERC-CREATE, Canada Research Chair
Date Deposited: 23 Nov 2018 10:20
Last Modified: 24 Nov 2018 01:20
PolyPublie URL: https://publications.polymtl.ca/3490/
Document issued by the official publisher
Journal Title: Scientific Reports (vol. 5)
Publisher: Nature Publishing Group
Official URL: https://doi.org/10.1038/srep11943

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