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Analytical technologies for influenza virus-like particle candidate vaccines: challenges and emerging approaches

Christine M. Thompson, Emma Petiot, Alexandre Lennaertz, Olivier Henry and Amine A. Kamen

Article (2013)

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Influenza virus-like particle vaccines are one of the most promising ways to respond to the threat of future influenza pandemics. VLPs are composed of viral antigens but lack nucleic acids making them non-infectious which limit the risk of recombination with wild-type strains. By taking advantage of the advancements in cell culture technologies, the process from strain identification to manufacturing has the potential to be completed rapidly and easily at large scales. After closely reviewing the current research done on influenza VLPs, it is evident that the development of quantification methods has been consistently overlooked. VLP quantification at all stages of the production process has been left to rely on current influenza quantification methods (i.e. Hemagglutination assay (HA), Single Radial Immunodiffusion assay (SRID), NA enzymatic activity assays, Western blot, Electron Microscopy). These are analytical methods developed decades ago for influenza virions and final bulk influenza vaccines. Although these methods are time-consuming and cumbersome they have been sufficient for the characterization of final purified material. Nevertheless, these analytical methods are impractical for in-line process monitoring because VLP concentration in crude samples generally falls out of the range of detection for these methods. This consequently impedes the development of robust influenza-VLP production and purification processes. Thus, development of functional process analytical techniques, applicable at every stage during production, that are compatible with different production platforms is in great need to assess, optimize and exploit the full potential of novel manufacturing platforms.

Uncontrolled Keywords

Virology; influenza virus-like particle (VLPs); assays; nucleic acids; process monitoring; analytical methods; Western blotting; virion; viral antigens; cell culture; enzyme activity; hemagglutination; manufacturing; electron microscopy; risk; purification methods; quantification; process analytical technologies; total particles, vaccines

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
5200 Microbiology > 5202 Virology
5200 Microbiology > 5204 Immunology
Department: Department of Chemical Engineering
Funders: CRSNG / NSERC - Discovery Grant
PolyPublie URL: https://publications.polymtl.ca/3448/
Journal Title: Virology Journal (vol. 10, no. 1)
Publisher: BioMed Central
DOI: 10.1186/1743-422x-10-141
Official URL: https://doi.org/10.1186/1743-422x-10-141
Date Deposited: 17 Jan 2019 14:08
Last Modified: 09 Apr 2024 01:06
Cite in APA 7: Thompson, C. M., Petiot, E., Lennaertz, A., Henry, O., & Kamen, A. A. (2013). Analytical technologies for influenza virus-like particle candidate vaccines: challenges and emerging approaches. Virology Journal, 10(1). https://doi.org/10.1186/1743-422x-10-141


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