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Chitosan rate of uptake in HEK293 cells is influenced by soluble versus microparticle state and enhanced by serum-induced cell metabolism and lactate-based media acidification

Caroline D. Hoemann, Jessica Guzmán-Morales, Nicolas Tran-Khanh, Geneviève Lavallée, Mario Jolicoeur and Marc Lavertu

Article (2013)

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Cite this document: Hoemann, C. D., Guzmán-Morales, J., Tran-Khanh, N., Lavallée, G., Jolicoeur, M. & Lavertu, M. (2013). Chitosan rate of uptake in HEK293 cells is influenced by soluble versus microparticle state and enhanced by serum-induced cell metabolism and lactate-based media acidification. Molecules, 18(1), p. 1015-1035. doi:10.3390/molecules18011015
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Abstract

Chitosan is a biocompatible polysaccharide composed of glucosamine and N-acetylglucosamine. The polymer has a unique behavior of fluctuating between soluble chains at pH 6 and insoluble microparticles at pH 7. The purpose of this study was to test the hypothesis that chitosan structure, solubility state, and serum influence the rate of cell uptake. Chitosans with 80% and 95% degree of deacetylation (medium and low viscosity) were tagged with rhodamine and analyzed for particle size, media solubility, and uptake by HEK293 epithelial cells using live confocal microscopy and flow cytometry. In media pH 7.4 with or without 10% serum, chitosans fully precipitated into 0.5 to 1.4 mu m diameter microparticles with a slight negative charge. During 24 h of culture in serum-free medium, chitosan particles remained extracellular. In cultures with serum, particles were taken up into intracellular vesicles in a serum dose-dependent manner. Opsonization of chitosan with serum, or replacement of serum by epidermal growth factor (EGF) failed to mediate serum-free chitosan particle uptake. Serum stimulated cells to acidify the media, partly by lactate generation. Media acidified to pH 6.5 by 7 mM lactate maintained 50% of chitosan in the soluble fraction, and led to minor uniform serum-free uptake in small vesicles. Conclusion: Media acidification mediates minor in vitro uptake of non-biofouled soluble chitosan chains, while serum-biofouled insoluble chitosan microparticles require sustained serum exposure to generate energy required for macropinocytosis.

Uncontrolled Keywords

EGF; HEK293 Cells; Serum; Epithelial Cells; Cells; Polysaccharides; Rhodamines; Acetylglucosamine; EGF Family of Proteins; Polymers; Glucosamine; Lactates; Chitosan; Particle Size; Viscosity; Solubility; Metabolism; Flow Cytometry; In Vitro Techniques; Microscopy, Confocal; chitosan; HEK293 cells; lactate; serum; confocal microscopy; microparticle

Open Access document in PolyPublie
Subjects: 1800 Génie chimique > 1800 Génie chimique
1900 Génie biomédical > 1900 Génie biomédical
Department: Département de génie chimique
Research Center: Non applicable
Funders: CRSNG / NSERC, Canadian Institutes of Health Research (CIHR), Fonds de la recherche Santé Québec (FRQS), Groupe de recherche en sciences et technologies biomédicales
Grant number: CIHR MOP 185810
Date Deposited: 15 Jan 2019 12:48
Last Modified: 16 Jan 2019 01:20
PolyPublie URL: https://publications.polymtl.ca/3441/
Document issued by the official publisher
Journal Title: Molecules (vol. 18, no. 1)
Publisher: MDPI
Official URL: https://doi.org/10.3390/molecules18011015

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