<  Back to the Polytechnique Montréal portal

Chitosan rate of uptake in HEK293 cells is influenced by soluble versus microparticle state and enhanced by serum-induced cell metabolism and lactate-based media acidification

Caroline D. Hoemann, Jessica Guzmán-Morales, Nicolas Tran-Khanh, Geneviève Lavallée, Mario Jolicoeur and Marc Lavertu

Article (2013)

Open Acess document in PolyPublie and at official publisher
Open Access to the full text of this document
Published Version
Terms of Use: Creative Commons Attribution
Download (867kB)
Show abstract
Hide abstract


Chitosan is a biocompatible polysaccharide composed of glucosamine and N-acetylglucosamine. The polymer has a unique behavior of fluctuating between soluble chains at pH 6 and insoluble microparticles at pH 7. The purpose of this study was to test the hypothesis that chitosan structure, solubility state, and serum influence the rate of cell uptake. Chitosans with 80% and 95% degree of deacetylation (medium and low viscosity) were tagged with rhodamine and analyzed for particle size, media solubility, and uptake by HEK293 epithelial cells using live confocal microscopy and flow cytometry. In media pH 7.4 with or without 10% serum, chitosans fully precipitated into 0.5 to 1.4 mu m diameter microparticles with a slight negative charge. During 24 h of culture in serum-free medium, chitosan particles remained extracellular. In cultures with serum, particles were taken up into intracellular vesicles in a serum dose-dependent manner. Opsonization of chitosan with serum, or replacement of serum by epidermal growth factor (EGF) failed to mediate serum-free chitosan particle uptake. Serum stimulated cells to acidify the media, partly by lactate generation. Media acidified to pH 6.5 by 7 mM lactate maintained 50% of chitosan in the soluble fraction, and led to minor uniform serum-free uptake in small vesicles. Conclusion: Media acidification mediates minor in vitro uptake of non-biofouled soluble chitosan chains, while serum-biofouled insoluble chitosan microparticles require sustained serum exposure to generate energy required for macropinocytosis.

Uncontrolled Keywords

EGF; HEK293 Cells; Serum; Epithelial Cells; Cells; Polysaccharides; Rhodamines; Acetylglucosamine; EGF Family of Proteins; Polymers; Glucosamine; Lactates; Chitosan; Particle Size; Viscosity; Solubility; Metabolism; Flow Cytometry; In Vitro Techniques; Microscopy, Confocal; chitosan; HEK293 cells; lactate; serum; confocal microscopy; microparticle

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
1900 Biomedical engineering > 1900 Biomedical engineering
Department: Department of Chemical Engineering
Funders: CRSNG / NSERC, Canadian Institutes of Health Research (CIHR), Fonds de la recherche Santé Québec (FRQS), Groupe de recherche en sciences et technologies biomédicales
Grant number: CIHR MOP 185810
PolyPublie URL: https://publications.polymtl.ca/3441/
Journal Title: Molecules (vol. 18, no. 1)
Publisher: MDPI
DOI: 10.3390/molecules18011015
Official URL: https://doi.org/10.3390/molecules18011015
Date Deposited: 15 Jan 2019 12:48
Last Modified: 31 Oct 2023 11:58
Cite in APA 7: Hoemann, C. D., Guzmán-Morales, J., Tran-Khanh, N., Lavallée, G., Jolicoeur, M., & Lavertu, M. (2013). Chitosan rate of uptake in HEK293 cells is influenced by soluble versus microparticle state and enhanced by serum-induced cell metabolism and lactate-based media acidification. Molecules, 18(1), 1015-1035. https://doi.org/10.3390/molecules18011015


Total downloads

Downloads per month in the last year

Origin of downloads


Repository Staff Only

View Item View Item