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Subchondral pre-solidified chitosan/blood implants elicit reproducible early osteochondral wound-repair responses including neutrophil and stromal cell chemotaxis, bone resorption and repair, enhanced repair tissue integration and delayed matrix deposition

Charles-Hubert Lafantaisie-Favreau, Jessica Guzmán-Morales, Jun Sun, Gaoping Chen, Adam Harris, Thomas D. Smith, Alberto Carli, Janet Henderson, William D. Stanish and Caroline D. Hoemann

Article (2013)

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Abstract

Background: In this study we evaluated a novel approach to guide the bone marrow-driven articular cartilage repair response in skeletally aged rabbits. We hypothesized that dispersed chitosan particles implanted close to the bone marrow degrade in situ in a molecular mass-dependent manner, and attract more stromal cells to the site in aged rabbits compared to the blood clot in untreated controls. Methods: Three microdrill hole defects, 1.4 mm diameter and 2 mm deep, were created in both knee trochlea of 30 month-old New Zealand White rabbits. Each of 3 isotonic chitosan solutions (150, 40, 10 kDa, 80% degree of deaceylation, with fluorescent chitosan tracer) was mixed with autologous rabbit whole blood, clotted with Tissue Factor to form cylindrical implants, and press-fit in drill holes in the left knee while contralateral holes received Tissue Factor or no treatment. At day 1 or day 21 post-operative, defects were analyzed by micro-computed tomography, histomorphometry and stereology for bone and soft tissue repair. Results: All 3 implants filled the top of defects at day 1 and were partly degraded in situ at 21 days post-operative. All implants attracted neutrophils, osteoclasts and abundant bone marrow-derived stromal cells, stimulated bone resorption followed by new woven bone repair (bone remodeling) and promoted repair tissue-bone integration. 150 kDa chitosan implant was less degraded, and elicited more apoptotic neutrophils and bone resorption than 10 kDa chitosan implant. Drilled controls elicited a poorly integrated fibrous or fibrocartilaginous tissue. Conclusions: Pre-solidified implants elicit stromal cells and vigorous bone plate remodeling through a phase involving neutrophil chemotaxis. Pre-solidified chitosan implants are tunable by molecular mass, and could be beneficial for augmented marrow stimulation therapy if the recruited stromal cells can progress to bone and cartilage repair.

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Subjects: 1800 Chemical engineering > 1800 Chemical engineering
1900 Biomedical engineering > 1902 Biomedical materials
2000 Materials science and technology > 2006 Biomaterials
9000 Health sciences > 9000 Health sciences
Department: Department of Chemical Engineering
Funders: CRSNG / NSERC, Canadian Institutes of Health Research (CIHR), Fonds de recherche Québec (FRSQ, CDH, FQRNT, JGM), Canadian Arthritis Network (AH, TDS), BiosSyntech Inc.
Grant number: STPGP 365025, 185810-BME
PolyPublie URL: https://publications.polymtl.ca/3427/
Journal Title: BMC Musculoskeletal Disorders (vol. 14, no. 1)
Publisher: BioMed Central Ltd
DOI: 10.1186/1471-2474-14-27
Official URL: https://doi.org/10.1186/1471-2474-14-27
Date Deposited: 05 Dec 2018 16:43
Last Modified: 10 Apr 2025 05:51
Cite in APA 7: Lafantaisie-Favreau, C.-H., Guzmán-Morales, J., Sun, J., Chen, G., Harris, A., Smith, T. D., Carli, A., Henderson, J., Stanish, W. D., & Hoemann, C. D. (2013). Subchondral pre-solidified chitosan/blood implants elicit reproducible early osteochondral wound-repair responses including neutrophil and stromal cell chemotaxis, bone resorption and repair, enhanced repair tissue integration and delayed matrix deposition. BMC Musculoskeletal Disorders, 14(1). https://doi.org/10.1186/1471-2474-14-27

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