Conception d'un adaptateur moléculaire pour l'immobilisation de facteurs de croissance sur un substrat de collagène

Collagen-based biomaterials have attracted a lot of interest in the field of tissue engineering. Their functionalization can be easily performed using chimeric proteins composed of a biomolecule, typically a growth factor, that is fused to a collagen-binding domain. This functionalization may provide biomaterials with mitogenic and anti-apoptotic properties, and influence the cellular fate within the implant. Many studies have been conducted to this end, in various fields of regenerative medicine, such as wound healing and bone regeneration. We chose to tether coil-tagged growth factors (Ecoil-GF) on a gelatin substrate using a molecular adaptor consisting of the collagen-binding domain of fibronectin, fused to the complementary coil (CBD-Kcoil). E and K coils interacted through coiled-coil interaction in a specific manner with high affinity, which enabled the formation of a ternary complex between the gelatin substrate, the molecular adapter CBD-Kcoil and the growth factor Ecoil-GF. We have produced and purified an Ecoil-tagged basic fibroblast growth factor (Ecoil-bFGF), and we have compared its tethering with that of the epidermal growth factor, using the molecular adaptor CBD-Kcoil.