Cécile Darviot, Bryan Gosselin, Flavie Martin, Sergiy Patskovsky, Ivan Jabin, Gilles Bruylants, Dominique Trudel et Michel Meunier
Article de revue (2024)
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Abstract
Reliable protein detection methods are vital for advancing biological research and medical diagnostics. While immunohistochemistry and immunofluorescence are commonly employed, their limitations underscore the necessity for alternative approaches. This study introduces immunoplasmonic labelling, utilizing plasmonic nanoparticles (NPs), specifically designed gold and gold–silver alloy NPs (Au:Ag NPs), for multiplexed and quantitative protein detection. These NPs, when coupled with antibodies targeting proteins of interest, enable accurate counting and evaluation of protein expression levels while overcoming issues such as autofluorescence. In this study, we compare two nanoparticle functionalization strategies—one coating based on thiolated PEG and one coating based on calix[4]arenes—on gold and gold–silver alloy nanoparticles of varying sizes. Overall results tend to demonstrate a greater versatility for the calix[4]arene-based coating. With this coating and using the classical EDC/sulfo-NHS cross-linking procedure, we also demonstrate the successful multiplexed immunolabelling of Her2, CD44, and EpCAM in breast cancer cell lines (SK-BR-3 and MDA-MB-231). Furthermore, we introduce a user-friendly software for automatic NP detection and classification by colour, providing a promising proof-of-concept for the practical application of immunoplasmonic techniques in the quantitative analysis of biopsies in the clinical setting.
Sujet(s): |
1900 Génie biomédical > 1900 Génie biomédical 1900 Génie biomédical > 1901 Technologie biomédicale 3100 Physique > 3100 Physique |
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Département: | Département de génie physique |
Organismes subventionnaires: | NSERC / CRSNG, Fonds de Recherche du Québec - Santé, Institut TransMedTech, Canadian Institutes of Health Research |
URL de PolyPublie: | https://publications.polymtl.ca/58790/ |
Titre de la revue: | Nanoscale Advances (vol. 2024) |
Maison d'édition: | The Royal Society of Chemistry |
DOI: | 10.1039/d4na00052h |
URL officielle: | https://doi.org/10.1039/d4na00052h |
Date du dépôt: | 30 juil. 2024 16:09 |
Dernière modification: | 25 sept. 2024 16:51 |
Citer en APA 7: | Darviot, C., Gosselin, B., Martin, F., Patskovsky, S., Jabin, I., Bruylants, G., Trudel, D., & Meunier, M. (2024). Multiplexed immunolabelling of cancer using bioconjugated plasmonic gold–silver alloy nanoparticles. Nanoscale Advances, 2024, 9 pages. https://doi.org/10.1039/d4na00052h |
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