A study of indole alkaloid production by surface immobilized Catharanthus Roseus plant cells

Natural products from plants -- Plant tissue and cell culture -- Project objectives -- In vitro techniques -- The plant cell -- Cell line generation -- Large scale cultures -- Indole alkaloids from catharanthus roseus -- Production techniques -- Production techniques -- Alternative methods -- Materials and methods -- Maintenance of the plan cell line -- Alkloid production regimes -- Surface immobilization of plant cells -- Biomass determination -- Indole alkaloid analysis -- Results -- Initial growth studies of catharanthus roseus cell cultures -- Growth of immobilized c.roseus cultures -- Development of a 2-stage culture regime -- Alkaloid studies in suspension cultures -- Alkaloid production studies in immobilized cultures -- Suspension culture growth -- Immobilized culture growth -- Alkaloid production in suspension cultures -- Alkaloid production in immobilized cultures.