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Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model

Wiam El Kheir, Alexandre Dumais, Maude Beaudoin, Bernard Marcos, Nick Virgilio, Benoît Paquette, Nathalie Faucheux and Marc-Antoine Lauzon

Article (2023)

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Abstract

Introduction: Extensive investigation has been undertaken regarding drug delivery systems for the management of glioblastoma multiforme (GBM). The infiltrative behavior of GBM cells within the brain tissue is primarily attributed to their heterogeneity, the movement of interstitial fluid (IFF), and the presence of chemokines. These factors contribute to the limited effectiveness of current conventional treatments. To address the dissemination of GBM cells, a proposed therapeutic approach involves utilizing a controlled release gradient of CXC-chemokine-ligand-12 (CXCL12). However, the impact of IFF on GBM cell migration within the brain underscores its critical importance as a significant parameter, which, surprisingly, has not been extensively studied in the context of localized drug delivery targeting the brain.

Methods: Hydrogels are known for their inherent capacity to entrap various agents and exert precise control over their subsequent release. In the present investigation, we aimed to elucidate the release kinetics of CXCL12, whether in its free form or encapsulated within nanoparticles, from alginate-based hydrogels, both under static and dynamic conditions. To investigate the impact of convective forces mimicking the interstitial fluid flow (IFF) within the peritumoral environment of the brain, a three-dimensional in vitro model was developed. This model enabled the evaluation of CXCL12 release as a function of time and position, specifically accounting for the contribution of simulated IFF on the release behavior.

Results: We first demonstrated that the release kinetic profiles under static culture conditions were independent of the initial mass loading and the predominant phenomenon occurring was diffusion. Subsequently, we investigated the release of CXCL12, which was loaded into Alginate/Chitosan-Nanoparticles (Alg/Chit-NPs) and embedded within an alginate hydrogel matrix. Mathematical modeling results also indicated the presence of electrostatic interactions between alginate and CXCL12. The Alg/Chit-NPs effectively slowed down the initial burst release, leading to a reduction in the diffusion coefficient of CXCL12. To further study the release behavior, we developed a perfusion bioreactor with a unique culture chamber designed to recapitulate the peritumoral environment and varied the fluid flow rates at 0.5 µL/min, 3 µL/min, 6.5 µL/min, and 10 µL/min. As the flow rate increased, the cumulative amount of released CXCL12 also increased for all three initial mass loadings. Beyond 3 µL/min, convection became the dominant mechanism governing CXCL12 release, whereas below this threshold, diffusion played a more prominent role.

Conclusion: The indirect perfusion flow had a crucial impact on CXCL12 release and distribution inside the hydrogel in and against its direction. This system highlights the importance of considering the IFF in brain targeting delivery system and will be used in the future to study GBM cell behaviors in response to CXCL12 gradient.

Uncontrolled Keywords

glioblastoma multiforme; nanoparticles; chemokines gradient; kinetic release; mathematical modeling; 3D delivery system; dynamic culture conditions; perfusion bioreactor

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
1800 Chemical engineering > 1802 Biochemical engineering
1900 Biomedical engineering > 1900 Biomedical engineering
Department: Department of Chemical Engineering
Funders: New Frontiers in Research Fund, Fonds de recherche du Québec – Nature et technologies
Grant number: NFRFE-2018-00764, 299713
PolyPublie URL: https://publications.polymtl.ca/55813/
Journal Title: Frontiers in Drug Delivery (vol. 3)
Publisher: Frontiers Media
DOI: 10.3389/fddev.2023.1227776
Official URL: https://doi.org/10.3389/fddev.2023.1227776
Date Deposited: 23 Jan 2024 17:46
Last Modified: 05 Apr 2024 23:24
Cite in APA 7: Kheir, W. E., Dumais, A., Beaudoin, M., Marcos, B., Virgilio, N., Paquette, B., Faucheux, N., & Lauzon, M.-A. (2023). Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model. Frontiers in Drug Delivery, 3, 1227776 (18 pages). https://doi.org/10.3389/fddev.2023.1227776

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