<  Back to the Polytechnique Montréal portal

Pixelated microfluidics for drug screening on tumour spheroids and ex vivo microdissected tumour explants

Dina Dorrigiv, Pierre-Alexandre F. Goyette, Amélie St-Georges-Robillard, Anne-Marie Mes-Masson and Thomas Gervais

Article (2023)

Open Acess document in PolyPublie and at official publisher
Open Access to the full text of this document
Published Version
Terms of Use: Creative Commons Attribution
Download (5MB)
Show abstract
Hide abstract


Anticancer drugs have the lowest success rate of approval in drug development programs. Thus, preclinical assays that closely predict the clinical responses to drugs are of utmost importance in both clinical oncology and pharmaceutical research. 3D tumour models preserve the tumoral architecture and are cost- and time-efficient. However, the short-term longevity, limited throughput, and limitations of live imaging of these models have so far driven researchers towards less realistic tumour models such as monolayer cell cultures. Here, we present an open-space microfluidic drug screening platform that enables the formation, culture, and multiplexed delivery of several reagents to various 3D tumour models, namely cancer cell line spheroids and ex vivo primary tumour fragments. Our platform utilizes a microfluidic pixelated chemical display that creates isolated adjacent flow sub-units of reagents, which we refer to as fluidic ‘pixels’, over tumour models in a contact-free fashion. Up to nine different treatment conditions can be tested over 144 samples in a single experiment. We provide a proof-of-concept application by staining fixed and live tumour models with multiple cellular dyes. Furthermore, we demonstrate that the response of the tumour models to biological stimuli can be assessed using the platform. Upscaling the microfluidic platform to larger areas can lead to higher throughputs, and thus will have a significant impact on developing treatments for cancer.

Uncontrolled Keywords

drug development; personalized medicine; preclinical assay; 3D tumour models; spheroids; ex vivo tumour explants; open-space microfluidics

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
9000 Health sciences > 9000 Health sciences
Department: Department of Engineering Physics
Institut de génie biomédical
Grant number: RGPIN 2020-06838
PolyPublie URL: https://publications.polymtl.ca/52584/
Journal Title: Cancers (vol. 15, no. 4)
Publisher: MDPI
DOI: 10.3390/cancers15041060
Official URL: https://doi.org/10.3390/cancers15041060
Date Deposited: 18 Apr 2023 14:58
Last Modified: 08 Apr 2024 22:11
Cite in APA 7: Dorrigiv, D., Goyette, P.-A. F., St-Georges-Robillard, A., Mes-Masson, A.-M., & Gervais, T. (2023). Pixelated microfluidics for drug screening on tumour spheroids and ex vivo microdissected tumour explants. Cancers, 15(4), 1060 (20 pages). https://doi.org/10.3390/cancers15041060


Total downloads

Downloads per month in the last year

Origin of downloads


Repository Staff Only

View Item View Item