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The potential of N-rich plasma-polymerized ethylene (PPE:N) films for regulating the phenotype of the nucleus pulposus

Fackson Mwale, Alain Petit, Hong Tian Wang, Laura M. Epure, Pierre-Luc Girard-Lauriault, Jean A. Ouellet, Michael R. Wertheimer and John Antoniou

Article (2008)

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Abstract

We recently developed a nitrogen-rich plasma-polymerized biomaterial, designated "PPE:N" (N-doped plasma-polymerized ethylene) that is capable of suppressing cellular hypertrophy while promoting type I collagen and aggrecan expression in mesenchymal stem cells from osteoarthritis patients. We then hypothesized that these surfaces would form an ideal substrate on which the nucleus pulposus (NP) phenotype would be maintained. Recent evidence using microarrays showed that in young rats, the relative mRNA levels of glypican-3 (GPC3) and pleiotrophin binding factor (PTN) were significantly higher in nucleus pulposus (NP) compared to annulus fibrosus (AF) and articular cartilage. Furthermore, vimentin (VIM) mRNA levels were higher in NP versus articular cartilage. In contrast, the levels of expression of cartilage oligomeric matrix protein (COMP) and matrix gla protein precursor (MGP) were lower in NP compared to articular cartilage. The objective of this study was to compare the expression profiles of these genes in NP cells from fetal bovine lumbar discs when cultured on either commercial polystyrene (PS) tissue culture dishes or on PPE:N with time. We found that the expression of these genes varies with the concentration of N ([N]). More specifically, the expression of several genes of NP was sensitive to [N], with a decrease of GPC3, VIM, PTN, and MGP in function of decreasing [N]. The expression of aggrecan, collagen type I, and collagen type II was also studied: no significant differences were observed in the cells on different surfaces with different culture time. The results support the concept that PPE:N may be a suitable scaffold for the culture of NP cells. Further studies are however necessary to better understand their effects on cellular phenotypes.

Subjects: 2000 Materials science and technology > 2004 Polymers and coatings
2000 Materials science and technology > 2010 Thin films/interfaces
3100 Physics > 3100 Physics
5100 Cell biology > 5105 Cell differentiation
Department: Department of Engineering Physics
Funders: IRSC/CIHR, AO Foundation, Switzerland, CRSNG/NSERC
PolyPublie URL: https://publications.polymtl.ca/5045/
Journal Title: Open Orthopaedics Journal (vol. 2, no. 1)
Publisher: Bentham Open
DOI: 10.2174/1874325000802010137
Official URL: https://doi.org/10.2174/1874325000802010137
Date Deposited: 14 Sep 2020 15:59
Last Modified: 08 Apr 2024 12:38
Cite in APA 7: Mwale, F., Petit, A., Tian Wang, H., Epure, L. M., Girard-Lauriault, P.-L., Ouellet, J. A., Wertheimer, M. R., & Antoniou, J. (2008). The potential of N-rich plasma-polymerized ethylene (PPE:N) films for regulating the phenotype of the nucleus pulposus. Open Orthopaedics Journal, 2(1), 137-144. https://doi.org/10.2174/1874325000802010137

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