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Can repeated in vivo micro-CT irradiation during adolescence alter bone microstructure, histomorphometry and longitudinal growth in a rodent model?

Tanvir Mustafy, Aurélie Benoit, Irène Londono, Florina Moldovan and Isabelle Villemure

Article (2018)

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Cite this document: Mustafy, T., Benoit, A., Londono, I., Moldovan, F. & Villemure, I. (2018). Can repeated in vivo micro-CT irradiation during adolescence alter bone microstructure, histomorphometry and longitudinal growth in a rodent model? PLOS One, 13(11). doi:10.1371/journal.pone.0207323
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Abstract

In vivo micro-computed tomography (micro-CT) can monitor longitudinal changes in bone mass and microstructure in small rodents but imposing high doses of radiation can damage the bone tissue. However, the effect of weekly micro-CT scanning during the adolescence on bone growth and architecture is still unknown. The right proximal tibia of male Sprague-Dawley rats randomized into three dose groups of 0.83, 1.65 and 2.47 Gy (n = 11/group) were CT scanned at weekly intervals from 4th to 12th week of age. The left tibia was used as a control and scanned only at the last time point. Bone marrow cells were investigated, bone growth rates and histomorphometric analyses were performed, and bone structural parameters were determined for both left and right tibiae. Radiation doses of 1.65 and 2.47 Gy affected bone marrow cells, heights of the proliferative and hypertrophic zones, and bone growth rates in the irradiated tibiae. For the 1.65 Gy group, irradiated tibiae resulted in lower BMD, Tb.Th, Tb.N and a higher Tb.Sp compared with the control tibiae. A decrease in BMD, BV/TV, Tb.Th, Tb.N and an increase in Tb.Sp were observed between the irradiated and control tibiae for the 2.47 Gy group. For cortical bone parameters, no effects were noticed for 1.65 and 0.83 Gy groups, but a lower Ct.Th was observed for 2.47 Gy group. Tibial bone development was adversely impacted and trabecular bone, together with bone marrow cells, were negatively affected by the 1.65 and 2.47 Gy radiation doses. Cortical bone microstructure was affected for 2.47 Gy group. However, bone development and morphometry were not affected for 0.83 Gy group. These findings can be used as a proof of concept for using the reasonable high-quality image acquisition under 0.83 Gy radiation doses during the adolescent period of rats without interfering with the bone development process.

Uncontrolled Keywords

Adolescent; Animals; Bone Development/radiation effects; Bone Marrow Cells/metabolism/pathology; Cancellous Bone/growth & development/pathology; Dose-Response Relationship, Radiation; Humans; Male; Mice; Rats, Sprague-Dawley; Tibia/growth & development/pathology; X-Ray Microtomography/adverse effects

Open Access document in PolyPublie
Department: Département de génie mécanique
Research Center: Non applicable
Funders: NSERC, CRC Program, NSERC/CREATE program
Date Deposited: 16 May 2022 17:02
Last Modified: 17 May 2022 01:20
PolyPublie URL: https://publications.polymtl.ca/5042/
Document issued by the official publisher
Journal Title: PLOS One (vol. 13, no. 11)
Publisher: PLOS
Official URL: https://doi.org/10.1371/journal.pone.0207323

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