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Antimicrobial efficacy of two surface barrier discharges with air plasma against in vitro biofilms

Rutger Matthes, Claudia Bender, Rabea Schlüter, Ina Koban, René Bussiahn, Stephan Reuter, Jürgen Lademann, Klaus-Dieter Weltmann and Axel Kramer

Article (2013)

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The treatment of infected wounds is one possible therapeutic aspect of plasma medicine. Chronic wounds are often associated with microbial biofilms which limit the efficacy of antiseptics. The present study investigates two different surface barrier discharges with air plasma to compare their efficacy against microbial biofilms with chlorhexidine digluconate solution (CHX) as representative of an important antibiofilm antiseptic. Pseudomonas aeruginosa SG81 and Staphylococcus epidermidis RP62A were cultivated on polycarbonate discs. The biofilms were treated for 30, 60, 150, 300 or 600 s with plasma or for 600 s with 0.1% CHX, respectively. After treatment, biofilms were dispensed by ultrasound and the antimicrobial effects were determined as difference in the number of the colony forming units by microbial culture. A high antimicrobial efficacy on biofilms of both plasma sources in comparison to CHX treatment was shown. The efficacy differs between the used strains and plasma sources. For illustration, the biofilms were examined under a scanning electron microscope before and after treatment. Additionally, cytotoxicity was determined by the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay with L929 mouse fibroblast cell line. The cell toxicity of the used plasma limits its applicability on human tissue to maximally 150 s. The emitted UV irradiance was measured to estimate whether UV could limit the application on human tissue at the given parameters. It was found that the UV emission is negligibly low. In conclusion, the results support the assumption that air plasma could be an option for therapy of chronic wounds.

Uncontrolled Keywords

Anti-Infective Agents/chemistry/*pharmacology; Anti-Infective Agents, Local/chemistry/pharmacology; Biofilms/*drug effects; Chlorhexidine/analogs & derivatives/chemistry; Pseudomonas aeruginosa/drug effects; Staphylococcus epidermidis/drug effects

Subjects: 3100 Physics > 3100 Physics
3100 Physics > 3107 Plasma physics
Department: Department of Engineering Physics
Funders: German Ministry of Education and Research (BMBF)
Grant number: 13N9779, 13N11181, 03Z2DN12
PolyPublie URL: https://publications.polymtl.ca/5024/
Journal Title: PLOS One (vol. 8, no. 7)
Publisher: PLOS
DOI: 10.1371/journal.pone.0070462
Official URL: https://doi.org/10.1371/journal.pone.0070462
Date Deposited: 11 May 2023 14:19
Last Modified: 10 Apr 2024 15:09
Cite in APA 7: Matthes, R., Bender, C., Schlüter, R., Koban, I., Bussiahn, R., Reuter, S., Lademann, J., Weltmann, K.-D., & Kramer, A. (2013). Antimicrobial efficacy of two surface barrier discharges with air plasma against in vitro biofilms. PLOS One, 8(7), e70462 (11 pages). https://doi.org/10.1371/journal.pone.0070462


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