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Characterization and standardization of tissue-simulating protoporphyrin IX optical phantoms

Mikael Marois, Jaime Bravo, Scott C. Davis and Stephen Chad Kanick

Article (2016)

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Abstract

Optical devices for measuring protoporphryin IX (PpIX) fluorescence in tissue are routinely validated by measurements in optical phantoms. Yet there exists limited data to form a consensus on the recipe for phantoms that both mimic the optical properties found in tissue and yield a reliable and stable relationship between PpIX concentration and the fluorescence remission intensity. This study characterizes the influence of multiple phantom components on PpIX fluorescence emission intensity, using Intralipid as the scattering source, bovine whole blood as the background absorber, and Tween as a surfactant to prevent PpIX aggregation. Optical measurements showed a linear proportionality (r > 0.99) between fluorescence intensity and PpIX concentration (0.1 to 10 mug/mL) over a range of Intralipid (1 to 2%) and whole blood (0.5 to 3%) for phantoms containing low surfactant (</= 0.1%), with fluorescence intensities and scattering and absorption properties stable for 5 h after mixing. The role of surfactant in PpIX phantoms was found to be complex, as aggregation was evident in aqueous nonturbid phantoms with no surfactant (0% Tween), and avoided in phantoms containing Intralipid as the scattering source with no additional or low amounts of added surfactant (</= 0.1% Tween). Conversely, phantoms containing higher surfactant content (>0.1% Tween) and whole blood showed interactions that distorted the fluorescence emissions.

Uncontrolled Keywords

*Models, Biological; Optical Imaging/*instrumentation; *Phantoms, Imaging; Protoporphyrins/*analysis; Spectrometry, Fluorescence/*instrumentation; Surface-Active Agents

Subjects: 3100 Physics > 3100 Physics
3100 Physics > 3110 Optics (see also Photon devices, 2505)
Department: Department of Engineering Physics
Funders: National Institutes of Health, National Institute of Neurological Disorders and Stroke (NINDS), National Cancer Institute (NCI)
Grant number: R01NS052274-06, K25CA164248-01
PolyPublie URL: https://publications.polymtl.ca/5016/
Journal Title: Journal of Biomedical Optics (vol. 21, no. 3)
Publisher: SPIE
DOI: 10.1117/1.jbo.21.3.035003
Official URL: https://doi.org/10.1117/1.jbo.21.3.035003
Date Deposited: 30 Sep 2021 15:51
Last Modified: 05 Apr 2024 18:26
Cite in APA 7: Marois, M., Bravo, J., Davis, S. C., & Kanick, S. C. (2016). Characterization and standardization of tissue-simulating protoporphyrin IX optical phantoms. Journal of Biomedical Optics, 21(3), 35003. https://doi.org/10.1117/1.jbo.21.3.035003

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