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In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography

I. Kestila, J. Thevenot, M. A. Finnila, S. S. Karhula, I. Hadjab, S. Kauppinen, M. Garon, E. Quenneville, M. Haapea, L. Rieppo, K. P. Pritzker, Michael D. Buschmann, H. J. Nieminen, S. Saarakkala

Article (2018)

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Abstract

OBJECTIVE: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (muCT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC. METHOD: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop muCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semi-automatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10%; zone 2: 10-40%; zone 3: 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6). RESULTS: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (P < 0.001) and less spherical (P < 0.001), respectively, in the OARSI grade 3-3.5 group compared to the OARSI grade 0-1.0 group. No statistically significant difference in chondron density between the OARSI grade groups was observed at different depths. CONCLUSION: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA).

Uncontrolled Keywords

Adult; Cartilage, Articular/*diagnostic imaging/pathology; Chondrocytes/*pathology; Female; Humans; Imaging, Three-Dimensional/*methods; In Vitro Techniques; Male; Middle Aged; Osteoarthritis/diagnostic imaging/pathology; X-Ray Microtomography/*methods; *3D analysis; *Hexamethyldisilazane; *Morphology; *Osteoarthritis; *Segmentation

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
Department: Institut de génie biomédical
Research Center: GRSTB - Biomedical Science and Technologies Research Centre
Funders: Academy of Finland, Sigrid Juselius Foundation, European Research Council under the European Union's Seventh Framework Programme, European Research Council
Grant number: 268378, 273571, 311586, FP/2007-2013, ERC Grant Agreement no. 336267
PolyPublie URL: https://publications.polymtl.ca/4968/
Journal Title: Osteoarthritis and Cartilage (vol. 26, no. 8)
Publisher: Elsevier
DOI: 10.1016/j.joca.2018.05.012
Official URL: https://doi.org/10.1016/j.joca.2018.05.012
Date Deposited: 20 Jan 2022 10:19
Last Modified: 10 Nov 2022 06:21
Cite in APA 7: Kestila, I., Thevenot, J., Finnila, M. A., Karhula, S. S., Hadjab, I., Kauppinen, S., Garon, M., Quenneville, E., Haapea, M., Rieppo, L., Pritzker, K. P., Buschmann, M. D., Nieminen, H. J., & Saarakkala, S. (2018). In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography. Osteoarthritis and Cartilage, 26(8), 1118-1126. https://doi.org/10.1016/j.joca.2018.05.012

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