I. Kestilä, J. Thevenot, M. A. Finnilä, S. S. Karhula, I. Hadjab, S. Kauppinen, M. Garon, E. Quenneville, M. Haapea, L. Rieppo, K. P. Pritzker, Michael D. Buschmann, H. J. Nieminen et S. Saarakkala
Article de revue (2018)
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Abstract
OBJECTIVE: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (muCT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC. METHOD: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop muCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semi-automatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10%; zone 2: 10-40%; zone 3: 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6). RESULTS: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (P < 0.001) and less spherical (P < 0.001), respectively, in the OARSI grade 3-3.5 group compared to the OARSI grade 0-1.0 group. No statistically significant difference in chondron density between the OARSI grade groups was observed at different depths. CONCLUSION: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA).
Mots clés
- Adult
- Cartilage, Articular/*diagnostic imaging/pathology
- Chondrocytes/*pathology
- Female
- Humans
- Imaging, Three-Dimensional/*methods
- In Vitro Techniques
- Male
- Middle Aged
- Osteoarthritis/diagnostic imaging/pathology
- X-Ray Microtomography/*methods
- *3D analysis
- *Hexamethyldisilazane
- *Morphology
- *Osteoarthritis
- *Segmentation
| Département: | Institut de génie biomédical |
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| Centre de recherche: | GRSTB - Centre de recherche en sciences et technologies biomédicales |
| Organismes subventionnaires: | Academy of Finland, Sigrid Juselius Foundation, European Research Council under the European Union's Seventh Framework Programme, European Research Council |
| Numéro de subvention: | 268378, 273571, 311586, FP/2007-2013, ERC Grant Agreement no. 336267 |
| URL de PolyPublie: | https://publications.polymtl.ca/4968/ |
| Titre de la revue: | Osteoarthritis and Cartilage (vol. 26, no 8) |
| Maison d'édition: | Elsevier |
| DOI: | 10.1016/j.joca.2018.05.012 |
| URL officielle: | https://doi.org/10.1016/j.joca.2018.05.012 |
| Date du dépôt: | 20 janv. 2022 10:19 |
| Dernière modification: | 09 avr. 2025 06:57 |
| Citer en APA 7: | Kestilä, I., Thevenot, J., Finnilä, M. A., Karhula, S. S., Hadjab, I., Kauppinen, S., Garon, M., Quenneville, E., Haapea, M., Rieppo, L., Pritzker, K. P., Buschmann, M. D., Nieminen, H. J., & Saarakkala, S. (2018). In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography. Osteoarthritis and Cartilage, 26(8), 1118-1126. https://doi.org/10.1016/j.joca.2018.05.012 |
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