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Real-time detection of Staphylococcus aureus using whispering gallery mode optical microdisks

Hala Ghali, Hicham Chibli, Jay L. Nadeau, Pablo Bianucci and Yves-Alain Peter

Article (2016)

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Whispering Gallery Mode (WGM) microresonators have recently been studied as a means to achieve real-time label-free detection of biological targets such as virus particles, specific DNA sequences, or proteins. Due to their high quality (Q) factors, WGM resonators can be highly sensitive. A biosensor also needs to be selective, requiring proper functionalization of its surface with the appropriate ligand that will attach the biomolecule of interest. In this paper, WGM microdisks are used as biosensors for detection of Staphylococcus aureus. The microdisks are functionalized with LysK, a phage protein specific for staphylococci at the genus level. A binding event on the surface shifts the resonance peak of the microdisk resonator towards longer wavelengths. This reactive shift can be used to estimate the surface density of bacteria that bind to the surface of the resonator. The limit of detection of a microdisk with a Q-factor around 10(4) is on the order of 5 pg/mL, corresponding to 20 cells. No binding of Escherichia coli to the resonators is seen, supporting the specificity of the functionalization scheme.

Uncontrolled Keywords

*Biosensing Techniques/instrumentation/methods; Sensitivity and Specificity; Silicon; *Staphylococcus aureus; bacterial sensing; optical microdisk; specific binding; surface functionalization

Subjects: 1900 Biomedical engineering > 1901 Biomedical technology
Department: Department of Engineering Physics
Funders: CRSNG/NSERC, Canadian Microelectronics Corporation (CMC) microsystems, Micro-Nano Technologies (MNT) financial assistance program
Grant number: Strategic grant 365207-2088
PolyPublie URL: https://publications.polymtl.ca/4886/
Journal Title: Biosensors (vol. 6, no. 2)
Publisher: MDPI
DOI: 10.3390/bios6020020
Official URL: https://doi.org/10.3390/bios6020020
Date Deposited: 30 Sep 2021 15:42
Last Modified: 11 Apr 2024 01:03
Cite in APA 7: Ghali, H., Chibli, H., Nadeau, J. L., Bianucci, P., & Peter, Y.-A. (2016). Real-time detection of Staphylococcus aureus using whispering gallery mode optical microdisks. Biosensors, 6(2), 1-9. https://doi.org/10.3390/bios6020020


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