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Optimizing Multiple Analyte Injections in Surface Plasmon Resonance Biosensors with Analytes having Different Refractive Index Increments

Massinissa Si Mehand, Balasubrahmanyan Srinivasan and Gregory De Crescenzo

Article (2015)

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Abstract

Surface plasmon resonance-based biosensors have been successfully applied to the study of the interactions between macromolecules and small molecular weight compounds. In an effort to increase the throughput of these SPR-based experiments, we have already proposed to inject multiple compounds simultaneously over the same surface. When specifically applied to small molecular weight compounds, such a strategy would however require prior knowledge of the refractive index increment of each compound in order to correctly interpret the recorded signal. An additional experiment is typically required to obtain this information. In this manuscript, we show that through the introduction of an additional global parameter corresponding to the ratio of the saturating signals associated with each molecule, the kinetic parameters could be identified with similar confidence intervals without any other experimentation.

Uncontrolled Keywords

Biosensing Techniques; Kinetics; Models, Theoretical; Molecular Weight; Refractometry; Sulfonamides; Surface Plasmon Resonance; Sulfonamides

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
Department: Department of Chemical Engineering
Funders: Canada Research Chair (CRC), Fonds québécois de la recherche sur la nature et les technologies (FQRNT), CRSNG / NSERC
PolyPublie URL: https://publications.polymtl.ca/3488/
Journal Title: Scientific Reports (vol. 5, no. 1)
Publisher: Nature Publishing Group
DOI: 10.1038/srep15855
Official URL: https://doi.org/10.1038/srep15855
Date Deposited: 22 Nov 2018 15:48
Last Modified: 19 Oct 2023 18:26
Cite in APA 7: Mehand, M. S., Srinivasan, B., & De Crescenzo, G. (2015). Optimizing Multiple Analyte Injections in Surface Plasmon Resonance Biosensors with Analytes having Different Refractive Index Increments. Scientific Reports, 5(1). https://doi.org/10.1038/srep15855

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