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Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion

Nabila Mighri, Jifu Mao, Frej Mighri, Abdellah Ajji and Mahmoud Rouabhia

Article (2015)

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Abstract

Designing scaffolds made from natural polymers may be highly attractive for tissue engineering strategies. We sought to produce and characterize chitosan-coated collagen membranes and to assess their efficacy in promoting chondrocyte adhesion, growth, and cytokine secretion. Porous collagen membranes were placed in chitosan solutions then crosslinked with glutaraldehyde vapor. Fourier transform infrared (FTIR) analyses showed elevated absorption at 1655 cm(-1) of the carbon-nitrogen (N=C) bonds formed by the reaction between the (NH2) of the chitosan and the (C=O) of the glutaraldehyde. A significant peak in the amide II region revealed a significant deacetylation of the chitosan. Scanning electron microscopy (SEM) images of the chitosan-coated membranes exhibited surface variations, with pore size ranging from 20 to 50 m. X-ray photoelectron spectroscopy (XPS) revealed a decreased C-C groups and an increased C-N/C-O groups due to the reaction between the carbon from the collagen and the NH2 from the chitosan. Increased rigidity of these membranes was also observed when comparing the chitosan-coated and uncoated membranes at dried conditions. However, under wet conditions, the chitosan coated collagen membranes showed lower rigidity as compared to dried conditions. Of great interest, the glutaraldehyde-crosslinked chitosan-coated collagen membranes promoted chondrocyte adhesion, growth, and interleukin (IL)-6 secretion. Overall results confirm the feasibility of using designed chitosan-coated collagen membranes in future applications, such as cartilage repair.

Uncontrolled Keywords

chitosan; chondrocytes; collagen; interleukin (IL)-6; membrane

Subjects: 1800 Chemical engineering > 1800 Chemical engineering
Department: Department of Chemical Engineering
Research Center: CREPEC - Center for Applied Research on Polymers and Composites
Funders: Fonds Emile Beaulieu, Laval University Foundation, Centre de recherche sur les systèmes polymères et composites à haute performance (CREPEC)
PolyPublie URL: https://publications.polymtl.ca/3477/
Journal Title: Materials (vol. 8, no. 11)
Publisher: MDPI
DOI: 10.3390/ma8115413
Official URL: https://doi.org/10.3390/ma8115413
Date Deposited: 06 Nov 2018 13:39
Last Modified: 11 May 2023 17:21
Cite in APA 7: Mighri, N., Mao, J., Mighri, F., Ajji, A., & Rouabhia, M. (2015). Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion. Materials, 8(11), 7673-7689. https://doi.org/10.3390/ma8115413

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