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Effect of Corneal Hydration on the Quality of the Femtosecond Laser Anterior Lamellar Cut

Ossama Nada, Anca Marian, Nicolas Tran-Khanh, Michael D. Buschmann, Michel Podtetenev, François Vidal, Santiago Costantino, Isabelle Brunette

Article (2014)

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The goal of this study was to assess the effect of corneal hydration on the quality of the femtosecond laser (FSL) anterior lamellar cut. The Visumax FSL was used to dissect an 8-mm-diameter corneal flap in 22 eye bank corneas showing various levels of hydration. The intended ablation depth was 220 mm in all eyes, which corresponded to the maximal depth available with this laser. After the cut, the achieved ablation depth was measured using optical coherence tomography images, flap separability was assessed by measuring the mean force generated to detach the flap, and stromal bed roughness was assessed by measuring the Haralick contrast level on the 1000 x scanning electron microscopy images of the ablated surfaces. The preoperative central corneal thickness ranged from 547 to 1104 mu m (mean +/- SEM: 833 +/- 30 mu m). A negative correlation was found between the level of corneal hydration and the ablation depth measured in the midperipheral cornea (r = -0.626, p = 0.003), the ablation being more superficial in more edematous corneas. The Haralick contrast also tended to increase as a function of corneal hydration ( r = 0.416, p = 0.061), suggesting that laser ablation in edematous corneas results in rougher stromal surfaces. These results support the hypothesis that the quality of the FSL lamellar cut decreases as the level of corneal hydration increases. Although FSL is still considered in the field as the tool of the future for corneal dissection, a better understanding of the limits of this tool will be needed before it can replace manual or automated stromal dissection techniques in hydrated corneas.

Uncontrolled Keywords

Cornea; Eyes; Lasers; Scanning electron microscopy; Tomography; Ophthalmic procedures; LASIK; Surgical and invasive medical procedures

Subjects: 1900 Biomedical engineering > 1901 Biomedical technology
9000 Health sciences > 9000 Health sciences
Department: Department of Chemical Engineering
Institut de génie biomédical
Research Center: GRSTB - Biomedical Science and Technologies Research Centre
Funders: Canadian Institutes of Health Research, Institut national canadien pour les aveugles (CNIB), Fonds de recherche en ophtalmologie de l'Université de Montréal (FROUM), Fonds de recherche du Québec – Santé - Research in Vision Network
PolyPublie URL: https://publications.polymtl.ca/3462/
Journal Title: PLOS One (vol. 9, no. 6)
Publisher: PLOS
DOI: 10.1371/journal.pone.0098852
Official URL: https://doi.org/10.1371/journal.pone.0098852
Date Deposited: 22 Nov 2018 15:30
Last Modified: 10 Nov 2022 15:06
Cite in APA 7: Nada, O., Marian, A., Tran-Khanh, N., Buschmann, M. D., Podtetenev, M., Vidal, F., Costantino, S., & Brunette, I. (2014). Effect of Corneal Hydration on the Quality of the Femtosecond Laser Anterior Lamellar Cut. PLOS One, 9(6). https://doi.org/10.1371/journal.pone.0098852


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