A Versatile Star PEG Grafting Method for the Generation of Nonfouling and Nonthrombogenic Surfaces

Abstract

Polyethylene glycol (PEG) grafting has a great potential to create nonfouling and nonthrombogenic surfaces, but present techniques lack versatility and stability. The present work aimed to develop a versatile PEG grafting method applicable to most biomaterial surfaces, by taking advantage of novel primary amine-rich plasma-polymerized coatings. Star-shaped PEG covalent binding was studied using static contact angle, X-ray photoelectron spectroscopy (XPS), and quartz crystal microbalance with dissipation monitoring (QCM-D). Fluorescence and QCM-D both confirmed strong reduction of protein adsorption when compared to plasma-polymerized coatings and pristine poly(ethyleneterephthalate) (PET). Moreover, almost no platelet adhesion was observed after 15 min perfusion in whole blood. Altogether, our results suggest that primary amine-rich plasma-polymerized coatings offer a promising stable and versatile method for PEG grafting in order to create nonfouling and nonthrombogenic surfaces and micropatterns.

Uncontrolled Keywords

Adsorption; Biocompatible Materials; Blood Platelets; Cardiovascular Diseases; Equipment Design; Humans; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Platelet Adhesiveness; Polyethylene Glycols; Polyethylene Terephthalates; Polymers; Proteins; Spectrometry, Fluorescence; Spectrophotometry; Surface Properties; Water; Biocompatible Materials; Polyethylene Terephthalates; Polymers; Proteins; Water; Polyethylene Glycols