<  Back to the Polytechnique Montréal portal

Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes

Alain Petit, Caroline N. Demers, Pierre-Luc Girard-Lauriault, Dorothy Stachura, Michael R. Wertheimer, John Antoniou and Fackson Mwale

Article (2011)

Open Acess document in PolyPublie and at official publisher
Open Access to the full text of this document
Published Version
Terms of Use: Creative Commons Attribution
Download (1MB)
Show abstract
Hide abstract


Background: Recent evidence indicates that osteoarthritis (OA) may be a systemic disease since mesenchymal stem cells (MSCs) from OA patients express type X collagen, a marker of late stage chondrocyte hypertrophy (associated with endochondral ossification). We recently showed that the expression of type X collagen was suppressed when MSCs from OA patients were cultured on nitrogen (N)-rich plasma polymer layers, which we call "PPE:N" (N-doped plasma-polymerized ethylene, containing up to 36 atomic percentage (at.%) of N.Methods: In the present study, we examined the expression of type X collagen in fetal bovine growth plate chondrocytes (containing hypertrophic chondrocytes) cultured on PPE:N. We also studied the effect of PPE: N on the expression of matrix molecules such as type II collagen and aggrecan, as well as on proteases (matrix metalloproteinase-13 (MMP-13) and molecules implicated in cell division (cyclin B2). Two other culture surfaces, "hydrophilic" polystyrene (PS, regular culture dishes) and nitrogen-containing cation polystyrene (Primaria (R)), were also investigated for comparison.Results: Results showed that type X collagen mRNA levels were suppressed when cultured for 4 days on PPE: N, suggesting that type X collagen is regulated similarly in hypertrophic chondrocytes and in human MSCs from OA patients. However, the levels of type X collagen mRNA almost returned to control value after 20 days in culture on these surfaces. Culture on the various surfaces had no significant effects on type II collagen, aggrecan, MMP-13, and cyclin B2 mRNA levels.Conclusion: Hypertrophy is diminished by culturing growth plate chondrocytes on nitrogen-rich surfaces, a mechanism that is beneficial for MSC chondrogenesis. Furthermore, one major advantage of such "intelligent surfaces" over recombinant growth factors for tissue engineering and cartilage repair is potentially large cost-saving.

Uncontrolled Keywords

Aggrecans; Animals; Cattle; Cell Culture Techniques; Cell Division; Cells, Cultured; Chondrocytes; Collagen Type II; Collagen Type X; Culture Media, Conditioned; Cyclin B2; Down-Regulation; Growth Plate; Hypertrophy; Matrix Metalloproteinase 13; Nitrogen; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Aggrecans; Collagen Type II; Collagen Type X; Culture Media, Conditioned; Cyclin B2; RNA, Messenger; Matrix Metalloproteinase 13; Nitrogen

Subjects: 1900 Biomedical engineering > 1900 Biomedical engineering
3100 Physics > 3100 Physics
Department: Department of Engineering Physics
Funders: CIHR, AO Foundation, CRSNG/NSERC
PolyPublie URL: https://publications.polymtl.ca/3405/
Journal Title: BioMedical Engineering OnLine (vol. 10, no. 4)
Publisher: BioMed Central
DOI: 10.1186/1475-925x-10-4
Official URL: https://doi.org/10.1186/1475-925x-10-4
Date Deposited: 10 Jan 2019 15:37
Last Modified: 10 Apr 2024 09:13
Cite in APA 7: Petit, A., Demers, C. N., Girard-Lauriault, P.-L., Stachura, D., Wertheimer, M. R., Antoniou, J., & Mwale, F. (2011). Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes. BioMedical Engineering OnLine, 10(4). https://doi.org/10.1186/1475-925x-10-4


Total downloads

Downloads per month in the last year

Origin of downloads


Repository Staff Only

View Item View Item